نتایج جستجو برای: cmcase assay
تعداد نتایج: 221846 فیلتر نتایج به سال:
The production, optimisation and partial characterisation of xylanases from newly isolated wild strains of Coprinellus disseminatus was performed in solid-state fermentation. Strains SH-1 and SH-2 showed high xylanase (727.78 and 227.99 IU/mL) with very low CMCase (0.925 and 0.660 IU/mL) and laccase (0.640 and 0.742 U/mL) activities at incubation time seven days, 37 degrees C and initial pH 6.4...
Aspergillus niger was used for cellulase production in submerged (SmF) and solid state fermentation (SSF). The maximum production of cellulase was obtained after 72 h of incubation in SSF and 96 h in Smf. The CMCase and FPase activities recorded in SSF were 8.89 and 3.56 U per g of dry mycelial bran (DBM), respectively. Where as in Smf the CMase & FPase activities were found to be 3.29 and 2.3 ...
Cellulose sausage cellulose casings are used extensively in the manufacture of sausages in meat packaging. After stripping the meat, spent asings mainly contain cellulose and residual meat juice with salt, nitrate and nitrite. Disposal of spent sausage casings has serious economic nd environmental concerns for the sausage industry. This work describes bioconversion of spent cellulose casings (S...
The strain of Penicillium janthinellum NCIM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24h followed by UV-irradiation for 3min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-d-glucose (SM3). These mutants were...
Different agricultural residues were considered in this study for their ability to support cellulolytic enzyme production by Aspergillus niger. A total of eleven agricultural residues including finger millet hulls, sorghum hulls, soybean hulls, groundnut husk, banana peels, corn stalk, cassava peels, sugarcane bagasse, saw dust, rice straw and sheanut cake were subjected to three pretreatment (...
A bacterial isolate from soil samples contaminated with decaying lignocellulosic wastes and effluents of cotton industry was isolated for cellulase production and was identified as Bacillus subtilis subsp. inaquosorum after morphological, biochemical and phlogenetic analysis. Under optimized solid state fermentation conditions maximum cellulase production 71.15 IU/ml was obtained with wheat bra...
The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (Trametes versicolor, Bjerkandera adusta, Ganoderma applanatum and Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feru...
Mushrooms degrade lignocellulosic biomass by releasing lignolytic and hydrolytic enzymes which convert material into soluble low molecular weight compounds are then absorbed as nutrients. In the present study, enzymatic activities of Pleurotus HK 37 during growth on Saba comorensis exocarps were evaluated. It was observed that, has ability to produce enzyme (Laccase) (Carboxymethyl cellulase, x...
To construct strains of the filamentous fungus Trichoderma reesei with low cellobiohydrolases while high endoglucanase activity, the P(cbh1)-eg3-T(cbh1) cassette was constructed and the coding sequence of the cellobiohydrolase I (CBHI) gene was replaced with the coding sequence of the eg3 gene by homologous recombination. Disruption of the cbh1 gene was confirmed by PCR, Southern dot blot and W...
The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cell...
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