The present study was carried out to investigate the effect of benazepril on corneal permeation in goat cornea and its effect on experimentally induced acute and chronic glaucoma in rabbits. Acute glaucoma was produced by i.v. infusion of 5% glucose (15 mL/kg) in rabbits, whereas chronic glaucoma was induced by injection of alpha-chymotrypsin into posterior chamber of rabbit eye. We studied the...

Plasma cholinesterase and erythrocyte acetylcholinesterase activities have a long history of use in monitoring both workers at risk of organophosphorus pesticide (OP) exposure and in investigating accidental exposures to OPs. On account of wide inter-individual variation, the establishment of unexposed, baseline enzyme activities is necessary for accurate interpretation. This paper describes th...

In search of potent inhibitors cholinesterase enzymes and antioxidant agents, synthetic derivatives dihydroquinazolin-4(1H)-one 1-38 were evaluated as potential anti-Alzheimer agents through in vitro acetylcholinesterase (AChE) butyrylcholinesterase (BChE) inhibitions radical (DPPH ABTS) scavenging activities. The (SAR) was mainly based on the different substituents at aryl part which showed a ...

By means of dibucaine inhibition (Kalow and Genest, 1957) it was found possible to differentiate three inherited serum cholinesterase phenotypes, those homozygous for the usual enzyme, homozygotes of the atypical enzyme and heterozygous individuals (Kalow and Staron, 1957). Harris and Whittaker (1961) then showed that sodium fluoride could be used to differentiate the serum cholinesterase varia...

Acetylcholinesterase (AChE) activity was measured in cholinergic and non-cholinergic neurons in the central nervous system of the leech. Intracellular AChE was assayed by pretreating intact ganglia with echothiophate to inhibit selectively extracellular enzyme. The concentration of intracellular AChE in cholinergic neurons was 3- to 24-fold higher than that in non-cholinergic cells. The propert...

Nepeta L. (Lamiaceae) species are belonging to aromatic and medicinal plants. Many biological activities of have been reported. In this study, memory-enhancing nuda ssp. essential oil was aimed be evaluated in laboratory rats treated with scopolamine. GC-MS/GC-FID system used identify composition. Memory performances the were tested using Y-maze radial-arm maze. Acetylcholinesterase enzyme acti...

Type A botulinum toxin was studied for its ability to inhibit the action of acetyl-cholinesterase. The chromogenic substrate, indophenyl acetate, was used for assay of enzyme activity. Inhibition of enzyme function was detected through use of both 6.6 x 10(-6) mg (20 ld(50)) and 6.6 x 10(-10) mg (2 x 10(-3)ld(50)) of type A botulinal toxin. Control assays were performed by use of both homologou...

1. Acetylcholinesterase from human erythrocytes was solubilized with Triton X-100 in strong salt solution and partially purified by (NH(4))(2)SO(4) fractionation. This preparation showed three main bands of enzyme activity after electrophoresis on polyacrylamide gel and incubation with either alpha-naphthyl acetate or acetylthiocholine as enzyme substrate. Two of the multiple forms were complet...

Plasma and brain cholinesterase activities were determined in three wild bird species to assess their exposure to organophosphate and carbamate insecticides which are used in agriculture and public health. In the present study, we used an electrometric method for measurement of cholinesterase activities in the plasma and whole brain of three indigenous wild birds commonly found in northern Iraq...

Cholinesterase is widely distributed in the animal kingdom, but its function apart from its involvement in the process of cholinergic transmission is unknown. Recent evidence indicates a close association between lipids and cholinesterase. Hyperlipaemia in humans.(Cucuinau etal., 1968)andrabbits (Kutty etal., 1973) is associated with increased serum cholinesterase activity. Cholinesterase has b...