Effects of Pi deficiency on photosynthesis ot isolated spinach chloroplasts were examined. The following observations were made: (1) Chloroplasts isolated in Pi-free media evolved oxygen in the light in the absence of added Pi until acid-extractable P, in the chloroplasts had decreased to 1 to 2.5 m M . This Pj was unavailable for photophosphorylation as shown by the inability of the chloroplas...

Leaves of C 4 plants contain two distinct types of photosynthetic cells, mesophyll (M) and bundle sheath (BS) which are quite different organized both structurally and functionally. There are three C 4 subgroups based on differences in decarboxylating mechanisms: NADP-ME, NAD-ME and PEP-CK type [1]. The BS chloroplasts in NADP-ME subtype are agranal with structures like stroma lamellae of C 3 c...

A nuclear recessive mutant of Arabidopsis thaliana, arc5, has been isolated in which there is no significant increase in chloroplast number during leaf mesophyll cell expansion and in which there are only 13 chloroplasts per mesophyll cell compared with 121 in wild-type cells. Mature arc5 chloroplasts in fully expanded mesophyll cells are 6-fold larger than in wild-type cells. A large proportio...

Lead ions are particularly dangerous to the photosynthetic apparatus, but little is known about the effects of trace metals, including Pb, on regulation of chloroplast redistribution. In this study a new effect of lead on chloroplast distribution patterns and movements was demonstrated in mesophyll cells of a small-sized aquatic angiosperm Lemna trisulca L. (star duckweed). An analysis of confo...

Isolated chloroplasts show a light-induced reversible increase in blue-green fluorescence (BGF), which is only dependent on NADPH changes. In the present communication, we report a time-resolved and spectral analysis of this BGF in reconstituted chloroplasts and intact isolated chloroplasts, in the dark and under actinic illumination. From these measurements we deduced the contribution of the d...

Liverworts belonging to the Class Hepaticae of the Division Bryophyta, are an excellent source of lower terpenoids. They possess intracellular oil bodies which are composed of lipophilic terpenoids and aromatic compounds. In this study, an attempt was made to identify the prenyllipid component that is localized to the chloroplasts of the liverwort, Heteroscyphus planus (Mitt.) Shiffner. The pre...

The chloroplasts of some members of the Dinophyceae are bounded by an envelope consisting of three membranes and having a mean thickness of 230 A. Within the chloroplast are arranged, in a more or less parallel manner, many lamellae normally composed of three apposed thylakoids, although the number of thylakoids often varies and may reach 30 in a single stack. By study of disintegrated chloropl...

In plants, chlorophylls and other tetrapyrroles are synthesized from a branched pathway that is located within chloroplasts. GUN4 (GENOMES UNCOUPLED 4) stimulates chlorophyll biosynthesis by activating Mg-chelatase, the enzyme that commits porphyrins to the chlorophyll branch. GUN4 stimulates Mg-chelatase by a mechanism that involves binding the ChlH subunit of Mg-chelatase, as well as a substr...

The Arabidopsis thaliana chloroplast protein ACCELERATED CELL DEATH2 (ACD2) modulates the amount of programmed cell death (PCD) triggered by Pseudomonas syringae and protoporphyrin IX (PPIX) treatment. In vitro, ACD2 can reduce red chlorophyll catabolite, a chlorophyll derivative. We find that ACD2 shields root protoplasts that lack chlorophyll from light- and PPIX-induced PCD. Thus, chlorophyl...

Translocon at the outer-envelope-membrane of chloroplasts 75 (Toc75) is the core component of the chloroplast protein import machinery. It belongs to the Omp85 family whose members exist in various Gram-negative bacteria, mitochondria, and chloroplasts of eukaryotes. Chloroplasts of Viridiplantae contain another Omp85 homolog called outer envelope protein 80 (OEP80), whose exact function is unk...