In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL(-1)) solely or in combination with cytokinins BAP and K...

Astragalus species are medicinal plants which produce valuable secondary metabolites, especially cycloartane-type glycosides. In this study, stem and leaf explants of Astragalus trojanus were subjected to different plant growth regulators, environmental conditions and media compositions to identify their callus responses. Stem and leaf explants were cultured in Murashige and Skoog (MS) and wood...

The indiscriminate use of commercial antimicrobial drugs has resulted in multiple drug resistance and adverse effects on the host. The present study was aimed to elucidate the effect of different biotic/abiotic elicitors on the growth as well as to enhance the metabolite production in leaf and epicotyl calli of Nigella sativa. Further, antibacterial activity of these callus extracts was evaluat...

The development of software applications that assist the radiographic evaluation of fracture healing could advance clinical diagnosis and expedite the identification of effective treatment strategies. A radiographic feature regularly used as an outcome measure for basic and clinical fracture healing research is new bone growth, or fracture callus. In this study, we developed OrthoRead, a portab...

Background and objectives: The Lamiaceae family is rich in favorable secondary metabolites which have different medicinal properties and also use in food, cosmetic and sanitary industry. Zataria multiflora Boiss. is an aromatic and bushy plant containing specific pharmaceutical components which is only distributed in certain regions of Iran. Tissue culture tec...

Comparison of natural plant and callus extracts of Solanum trilobatum L. was studied against two bacteria and fungi, for their antimicrobial activity using cup diffusion method. Various solvents such as chloroform, petroleum ether and ethanol were used. The leaf and stem segments of the plant were culturedon Murashige and S koog basal medium supplemented with various growth regulators. Maximum ...

Isoflavonoid contents of different plant parts and callus tissues of the Indian Kudzu, Pueraria tuberosa (Roxb.ex.Willd.) DC are presented. The initial cultures were slow growing, associated with browning of the tissues. The production of four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus cultures of P. tuberosa was studied by manipulating the plant growth regulators ...

Cytokinin affects the requirement for auxin of a strain of tobacco callus (Nicotiana tabacum) which is cytokinin-autotrophic when grown on Murashige and Skoog medium with 11.4 mum of indole-3-acetic acid but requires cytokinin 6-(3-methyl-2-butenylamino)purine (i(6) Ade) when grown on the same medium with <3 mum indole-3-acetic acid. As the exogenous concentration of cytokinin (i(6) Ade) is inc...

taxus    is an endangered plant which is the only commercial source of paclitaxel, which is used for treatment of ovary and breast cancer. as production of this valuable drug is a destructive process, other alternatives should be considered for its sustainable production. plant tissue and cell culture is a promising method for production of secondary metabolites. in order to optimize yew callus...