A cell suspension culture o f Catharanthus roseus was trea ted during the grow th phase w ith the nucleoside analogue 5-azacytidine, a powerful inducer o f cell d ifferen tia tion , as evidenced w ith animal and hum an cell lines. After induction the cell cu lture was fu rth er incubated in a production m edium in the absence o f the inducer for 10 days. T he extraction o f the freeze dried cel...

This paper is in line with the previous publication on the wood inhabiting Hyphomycetes from Gilan province (Iran). As a results, 22 taxa were identified which are listed as follows: Arthrinium state of Apiospora montagnei, Arthrinium saccharicola, Arthrobotrys sp., Berkleasmium sp., Clonostachys rosea, Cryptocoryneum sp., Cylindrocladium colhonii, Dendrostilbella sp., Dendryphion comosum, Endo...

Ammonia-lyase activity for L-phenylalanine, m-hydroxyphenylalanine and L-tyrosine was demonstrated in cell-free extracts ofSporobolomyces roseus. Cultures of this organism converted DL-[ring-14C]phenylalanine and L-[U-14C]tyrosine into the corresponding cinnamic acid. Tracer studies showed that these compounds were further metabolized to [14C]protocatechuic acid. Benzoic acid andp-hydroxybenzoi...

A particulate enzyme system from Micrococcus roseus R2J has been obtained which catalyzes the biosynthesis of a peptidoglycan. The preparation catalyzes the incorporation of L-threonine onto the e-amino group of lysine in the presence of transfer RNA @RNA), supernatant solution, and ATP. Crude l%-L-threonyl-tRNA will substitute for these additional requirements. The threonyl-tRNAs of this organ...

A kinetic metabolic model describing Catharanthus roseus hairy root growth and nutrition was developed. The metabolic network includes glycolysis, pentose-phosphate pathway, TCA cycle and the catabolic reactions leading to cell building blocks such as amino acids, organic acids, organic phosphates, lipids and structural hexoses. The central primary metabolic network was taken at pseudo-steady s...

The biocatalytic synthesis of xylooligosaccharides of daidzein was investigated using cultured cells of Catharanthus roseus and Aspergillus sp. β-xylosidase. The cultured cells of C. roseus converted daidzein into its 4'-O-β-glucoside, 7-O-β-glucoside, and 7-O-β-primeveroside, which was a new compound. The 7-O-β-primeveroside of daidzein was further xylosylated by Aspergillus sp. β-xylosidase t...

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

catharanthus roseus is a medicinal and ornamental plant with growing attention toward its economical value. cell suspension from c. roseus were treated with 0 to 60mm of cadmium nitrate for 1, 3 and 6 days, then cell viability was determined using trypan blue and mtt assay. cell morphology was investigated using 0, 10, 30 and 50 mm of cadmium nitrate as selected dose for 3 days. callus of c.ros...

Catharanthus roseus is a medicinal and ornamental plant with growing attention toward its economical value. Cell suspension from C. roseus were treated with 0 to 60mM of cadmium nitrate for 1, 3 and 6 days, then cell viability was determined using trypan blue and MTT assay. Cell morphology was investigated using 0, 10, 30 and 50 mM of cadmium nitrate as selected dose for 3 days. Callus of C.ros...