نتایج جستجو برای: and ns5a proteins

تعداد نتایج: 16874701  

Journal: :Virology 2000
S Satoh M Hirota T Noguchi M Hijikata H Handa K Shimotohno

Nonstructural 5A protein (NS5A) of hepatitis C virus (HCV) is localized in the cytoplasm although it has a functional nuclear localization signal. To clarify the determinant of NS5A cytoplasmic localization, various N- or C-terminal deleted NS5A mutants were generated and their subcellular localization was analyzed in cell lines after transient expression. N-terminal deleted forms of NS5A were ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1999
S L Tan H Nakao Y He S Vijaysri P Neddermann B L Jacobs B J Mayer M G Katze

Although hepatitis C virus (HCV) infection is an emerging global epidemic causing severe liver disorders, the molecular mechanisms of HCV pathogenesis remain elusive. The NS5A nonstructural protein of HCV contains several proline-rich sequences consistent with Src homology (SH) 3-binding sites found in cellular signaling molecules. Here, we demonstrate that NS5A specifically bound to growth fac...

2013
Elizabeth M Quezada Caroline M Kane

The Hepatitis C Virus RNA dependent RNA polymerase, NS5B, is stimulated by the NS5A protein in vitro. To explore this stimulatory mechanism, we compared the activity of a mutant of NS5B containing a deletion of the β-loop region with that of the full length NS5B in response to NS5A. While the NS5A protein does stimulate full length NS5B, NS5A does not stimulate the NS5B deletion mutant during e...

2013
Simon Reiss Christian Harak Inés Romero-Brey Danijela Radujkovic Rahel Klein Alessia Ruggieri Ilka Rebhan Ralf Bartenschlager Volker Lohmann

The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an essential host factor of hepatitis C virus (HCV) replication. PI4KIIIα catalyzes the synthesis of phosphatidylinositol 4-phosphate (PI4P) accumulating in HCV replicating cells due to enzyme activation resulting from its interaction with nonstructural protein 5A (NS5A). This study describes the interaction between PI4KIIIα...

Journal: :Journal of virology 2001
M Majumder A K Ghosh R Steele R Ray R B Ray

We have previously demonstrated that hepatitis C virus (HCV) NS5A protein promotes cell growth and transcriptionally regulates the p21/waf1 promoter, a downstream effector gene of p53. In this study, we investigated the molecular mechanism of NS5A-mediated transcriptional repression of p21/waf1. We observed that transcriptional repression of the p21/waf1 gene by NS5A is p53 dependent by using p...

Journal: :Journal of virology 2009
Robert A Love Oleg Brodsky Michael J Hickey Peter A Wells Ciarán N Cronin

A new protein expression vector design utilizing an N-terminal six-histidine tag and tobacco etch virus protease cleavage site upstream of the hepatitis C virus NS5A sequence has resulted in a more straightforward purification method and improved yields of purified NS5A domain I protein. High-resolution diffracting crystals of NS5A domain I (amino acids 33 to 202) [NS5A(33-202)] were obtained b...

Journal: :Journal of virology 2004
Carlos Coito Deborah L Diamond Petra Neddermann Marcus J Korth Michael G Katze

The hepatitis C virus NS5A protein plays a critical role in virus replication, conferring interferon resistance to the virus through perturbation of multiple intracellular signaling pathways. Since NS5A is a phosphoprotein, it is of considerable interest to understand the role of phosphorylation in NS5A function. In this report, we investigated the phosphorylation of NS5A by taking advantage of...

2017
Zhengyuan Xie Zhihua Xiao Fenfen Wang

The nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus (HCV) RNA genome is a multifunctional phosphoprotein. To analyse the influence of NS5A on apoptosis, we established an Hep-NS5A cell line (HepG2 cells that stably express NS5A) and induced apoptosis using tumour necrosis factor (TNF)-α. We utilised the MTT assay to detect cell viability, real-time quantitative polymerase...

2013
Silvia Dibenedetto David Cluet Pierre-Nicolas Stebe Véronique Baumle Jérémie Léault Raphaël Terreux Marc Bickle Benoit D. E. Chassey Ivan Mikaelian Pierre Colas Martin Spichty Michele Zoli Brian B. Rudkin

We previously identified a peptide aptamer (named R5G42) via functional selection for its capacity to slow cell proliferation. A yeast two-hybrid screen of human cDNA libraries, using R5G42 as "bait," allowed the identification of two binding proteins with very different functions: calcineurin A (CnA) (PP2B/PPP3CA), a protein phosphatase well characterized for its role in the immune response, a...

Journal: :Journal of virology 2006
Manuela Quintavalle Sonia Sambucini Chiara Di Pietro Raffaele De Francesco Petra Neddermann

Hepatitis C virus (HCV) has been the subject of intensive studies for nearly two decades. Nevertheless, some aspects of the virus life cycle are still a mystery. The HCV nonstructural protein 5A (NS5A) has been shown to be a modulator of cellular processes possibly required for the establishment of viral persistence. NS5A is heavily phosphorylated, and a switch between a basally phosphorylated ...

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