the leopard moth, zeuzera pyrina, is a key and worldwide pest damaging walnut as well as many other fruit trees during the larval stages. larvae were collected from infested twigs of walnut trees. midguts from larvae were removed under a stereomicroscope and their homogenates used as the enzyme source. larval midgut extracts showed an optimum activity at ph 8.0, 6.0 and 6.0 for ?-amylase, ?- an...

We coupled a kinetic procedure to a selective inhibiting method for determining amylase isoenzymes in biological samples, using 4-nitrophenylmaltopentaoside plus 4-nitrophenylmaltohexaoside as substrate and a wheat-germ selective inhibitor with the Gilford S-III spectrophotometer. On plotting remaining amylase activities/total amylase activities (R/T) vs pancreatic amylase activities/salivary a...

BACKGROUND This study was performed to investigate the impact of exogenous ghrelin on the pancreatic α-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine. METHODS Sprague-Dawley male rats (9 weeks old, 300 ± 10 g) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and 10.0 μg/kg body weight (BW), respecti...

24-h intravenous caerulein infusion studies in the rat were combined with in vitro amino acid incorporation studies followed by high-resolution separation of proteins by two-dimensional isoelectric focusing and SDS gel electrophoresis to study the extent to which persistent changes in the biosynthesis of exocrine pancreatic proteins are regulated by cholecystokinin-like peptides. Beginning in t...

Amylase expression in strain YBR differs in several respects from the standard mouse phenotype. The synthesis of salivary amylase is elevated twofold in YBR mice and the synthesis of pancreatic amylase is reduced to one-half the normal rate. We have compared the concentrations of amylase mRNA in the parotid, liver and pancreas of YBR mice with those in strains A/J and C3H. We observed differenc...

Porcine pancreatic alpha-amylase (PPA) was allowed to react with herbal extracts containing rosmarinic acid (RA) and purified RA. The derivatized enzyme-phytochemical mixtures obtained were characterized for residual amylase activity. These in vitro experiments showed that the amylase activity was inhibited in the presence of these phytochemicals. The extent of amylase inhibition correlated wit...

Background & Aim: Singhnad Guggulu is a traditional Ayurvedic formula for detoxifying and rejuvenating the joints. The present study was to investigate the α-amylase inhibition and antioxidant activities of aqueous extract of Singhnad Guggul (AESG). Experimental: α-Amylase inhibition assay by dinitro-salicylic acid method and antioxidant activity by ferric chelating and ferrous reducing assay....

Of alpha-amylases and other starch-digesting enzymes from bacteria and plants.ess was supplemented with bacterial and fungal amylases or scalded flour. Immunochemical characteristics of alpha-amylase sensitization.Alpha-amylase production and its activity was compared in two different. Bacterial amylases are resistant to high temperatures, they can active in wide pH.fungal amylascs lowest, liqu...

The clearance of pancreatic and salivary amylase relative to creatinine was measured in 26 control subjects and 22 patients with chronic pancreatitis. Control values for pancreatic amylase clearance (+/- SD) were 2.64 +/- 0.86% compared with 1.54 +/- 0.95% for salivary amylase. In chronic pancreatitis, pancreatic amylase clearance ratios were significantly higher than controls (P less than 0.00...

Bauhinia racemosa Lamk. (Caesalpiniaceae) is a small tree where bark and leaves are used extensively for the treatment of inflammation, headache, fever, malaria, skin infections, dysentery and diarrhea. The primary objective of this study was to evaluate the antioxidant, DNA cleavage and α-amylase inhibitory effects of the phenolic compounds: racemosol (BR-I), de-O-methyl racemosol (BR-II), 1,7...