In their attempt to deconstruct sex therapy and bury the remains, Binik and Meana (2009) ask “Will the ‘real’ sex therapist please stand up?” I am not sure about being “real,” but I was a sex therapist for 40 years until my retirement. I have treated many couples and individuals and also trained many therapists in the use of sex therapy. These trainees have come from a range of professional bac...

Transcription studies with highly purified potato cell nuclei in combination with a 'transcription-hybridization analysis' unequivocally demonstrate that the nucleus is the subcellular site where the entire process of PSTV replication takes place. Inhibition experiments with actinomycin D and alpha-amanitin furthermore suggest that the nuclear DNA-dependent RNA polymerases I and II are involved...

Cellular DNA-dependent RNA polymerase II (pol II) has been postulated to carry out RNA-dependent RNA replication and transcription of hepatitis delta virus (HDV) RNA, generating a full-length (1.7-kb) RNA genome and a subgenomic-length (0.8-kb) mRNA. However, the supporting evidence for this hypothesis was ambiguous because the previous experiments relied on DNA-templated transcription to initi...

Primary cultures of bovine adrenal chromaffin cells provide large quantities of a homogeneous population of target cells for nerve growth factor (NGF) and, thus, are a suitable system for studying the molecular mechanism of action of NGF. In this study, we have shown that NGF mediates the specific induction of the key enzymes in catecholamine biosynthesis, tyrosine hydroxylase (TH), dopamine-be...

By the special polygonal curve we meana simple closed curve, that is a polygone and moreover has edges parallel to axes. We continue the formalization of the Takeuti-Nakamura proof [21] of the Jordan curve theorem. In the paper we prove that the complement of the special polygonal curve consists of at least two components. With the theorem which has at most two components we completed the theor...

Mutations in the Caenorhabditis elegans RNA polymerase II AMA-1/RPB-1 subunit that cause α-amanitin resistance and/or developmental defects were isolated previously. We identified 12 of these mutations and mapped them onto the Saccharomyces cerevisiae RPB1 structure to provide insight into AMA-1 regions that are essential for development in a multicellular organism.