The objective is to evaluate in situ detection of living mycobacterium tuberculosis rRNA using Mycobacterium Tuberculosis Direct Assay (MTD) and its clinical significance in early diagnosis of extrapulmonary tuberculosis. 86 patients having been diagnosed to have extrapulmonary tuberculosis including tuberculous peritonitis (n=22), lymphatic tuberculosis (n=21), tuberculous meningitis (n=15), H...

the aim of this study was to analyse polar lipids of candida species isolated from ahwaz (iran) by fast atom bombardment mass spectrometry (fab ms). nine isolates of candida sp. were identified by growth at 45°c, production of chlamydoconidia on cornmeal agar, colonial colour on chromagar candida, germ tube production and id 32c kits. then polar lipids were extracted from freeze-dried cultures ...

Calf thymus histories comprising two fractions, one rich in lysine, the other having roughly equal amounts of lysine and arginine, Loligo testes histones rich in arginine, and salmine, are compared with respect to their amino acid compositions, and their staining properties when the proteins are fixed on filter paper. The three types of basic proteins; somatic, arginine-rich spermatid histones,...

Introduction: Mycobacterium tuberculosis is the second most infectious cause of death in adults globally and major health trouble Nepal. The diagnosis pulmonary mainly carried by acid-fast bacilli smear microscopy, culture on Lowenstein–Jensen media, GeneXpert MTB/RIF assay. This study aimed to evaluate Acid-Fast Bacilli stain used for tuberculosis.
 Methods: A cross-sectional was performe...

The effect of 20 kHz ultrasound on the viability of Cryptosporidium oocysts was investigated. More than 90% of the dispersed Cryptosporidium oocysts could be deactivated in about 1.5 min of continuous sonication. In order to apply this technique to large quantities of contaminated water, quantitative filtration and redispersion of Cryptosporidium oocysts were investigated and found to be easily...

Brucellosis is one of the most common zoonotic diseases in India. Growth of Brucella is slow and needs meticulous biochemical tests and antisera for confirmation of the isolates. In the present study modified cold ZN staining was tried on the broth cultures for early presumptive identification of Brucella growth. Blood cultures were attempted in 22 seropositive patients. In the five blood cultu...

Spindle cell pseudotumors may occur due to mycobacterial infection in immunocompromised hosts, particularly those with acquired immunodeficiency syndrome (AIDS). Most of the reported mycobacterial spindle cell pseudotumors were found in the lymph nodes. We report a case of spindle cell pseudotumor in a 37-year-old man with AIDS who presented with a firm nodule over his right arm. Histologically...

We hereby report a case of a 41-year-old woman with a history of liposuction of the right hip 6 months previously who presented to her dermatologist with bilateral recurring erythematous skin nodules. On palpation, 1 cm tender nodules were identified in the bilateral hip region in close approximation to the liposuction scars and a shave biopsy was performed from the lesions on the right side. T...

A cross-sectional study was conducted to investigate risk factors for sporadic Cryptosporidium infection in a paediatric population in Nigeria. Of 692 children, 134 (19·4%) were infected with Cryptosporidium oocysts. Cryptosporidium spp. were identified in 49 positive samples using PCR-restriction fragment length polymorphism and direct sequencing of the glycoprotein60 (GP60) gene. Generalized ...

BACKGROUND Cryptosporidium spp. is a coccidian parasite infected humans and animals. Prevalence rate of Cryptosporidium spp. infection associated with is some parameters such as sampling, age, season, country and contact to domestic animals. This study aimed to determine Cryptosporidium spp. Infection in humans and some animals in rural areas of Shushtar district from Khuzestan Province, south-...