There are differences between the EPA Method 365 and the APHA-AWWA-WEF's Standard Method 4500 with respect to the post-digestion treatment procedures of the persulfate-digested water. The effects on total phosphorus analysis of different post-digestion treatment procedures, such as neutralization and reacidification, and shaking/settling, were investigated in this study using the total phosphor...

A low molecular weight RNA was isolated from human cells infected with adenovirus 2 and its nucleotide sequence deduced to be ,G-G-G-C-A-C-U-C-U-U-C-C-G-U-G-G-UC-U-G-G-U-G-G-A-U-A-A-A-U-U-C-G-C-A-A-G-G-G-U-AU-C-A-U-G-G-C-(G-U-G-G-A-C-G-A-C-C)-G-G-G-G-U-UC-G-A-A-C-C-C-C-G-G-A-U-C-C-G-U-G-A-U-C-C-A-U-GC-G-G-U-U-A-C-C-G-U-C-C-G-C-C-G-C-C-C-G-U-G-C-GU-C-G-A-A-C-C-C-A-G-G-U-G-U-G-C-G-A-C-G-U-C-AG-A-...

A fluorometric assay for pepsin and pepsinogen was developed using enhanced green fluorescent protein (EGFP) as a substrate. Acid denaturation of EGFP resulted in a complete loss of fluorescence that was completely reversible on neutralization. In the proteolytic assay procedure, acid-denatured EGFP was digested by pepsin or activated pepsinogen. After neutralization, the remaining amount of un...

A large alkaline tide (up to 20 mmol l(-1) increase in bicarbonate concentration [HCO3-] with an accompanied increase in blood pH) has previously been reported for some carnivorous reptiles within 24 h after ingesting a large meal. This phenomenon has been attributed to the secretion of large amounts of H+ ions into the stomach, which is required for digestion of large prey items. To test the g...

Two of the most important Mexican plant-foods are tomato (Solanum lycopersicum L.) and husk (Physalis ixocarpa Brot.). In this study three objectives were followed: i) to evaluate bioaccessible phenolic compounds (PC) in T HT during upper gastrointestinal digestion, ii) vitro ferment indigestible fractions samples short-chain fatty acids (SCFA) production, iii) microbial metabolites, bioconvert...

a pancreatopeptidase (elastase) digest index was devised for a rapid and accurate estimation of protein quality. this index was calculated on the basis of all the amino acids released by an in-vitro elastase digestion, acid hydrolyses of same sample and the residue of enzyme hydrolyzed. the amino acids were determined by thin-layer chromatography. samples used were cooked white kidneybeans, coo...

A study was made of the enzymatic properties of crystalline desoxyribonuclease. The general effect of the crystalline enzyme on its specific substrate, thymus nucleic acid, was found to be essentially the same as described by previous workers for the digestive action of crude preparations of the enzyme. The digestive action consists mainly in splitting thymus nucleic acid into fragments approac...

A three-step in vitro procedure was developed to estimate intestinal digestion of proteins in ruminants. Dacron bags containing feed samples were suspended in the rumen for 16 h. Residue containing 15 mg of N after ruminal exposure was incubated for 1 h in 10 mL of a .1 N HCl solution containing 1 g/L of pepsin. After incubation, pH was neutralized with .5 mL of 1 N NaOH and 13.5 mL of a pH 7.8...

It has been reported that intravenous injection of nicotinic acid produces hypoglycemia in normal humans (l-4). A definite improvement in the carbohydrate tolerance of diabetic patients after administration of nicotinic acid has also been reported (4). It was therefore of interest to study the urinary excretion of nicotinic acid in diabetic patients. In the chemical estimation of nicotinic acid...

Nucleic acid extracted from environmental samples is an important analyte in instrumental analysis. With respect to the phenomenon which sodium tungstate promotes methane production anaerobic digestion, analyses of microbial community using RNA were conducted; however, we noticed that only concentration measured by absorbance measurement (NanoDrop) was remarkably high. Therefore, this study, DN...