Ruellia tuberosa (family Acanthaceae) is widely known in traditional medicine Asian countries for the treatment of diabetes and other diseases. Its roots were demonstrated to possess a hypoglycemic ability diabetic animal models. In this study, an original induced procedure was investigated establish hairy root (HR) from R. tuberosa. With aim increasing transformation rate, some factors (acetos...

Plant regeneration studies in cereals have been undertaken in immature embryos, scutellum and also in immature inflorescence tissue. The wheat mature embryos can also be employed for callusing and regeneration, as they are available throughout the year and have presently been employed for transformation studies. An efficient and reproducible method for Agrobacterium-mediated transformation of m...

Laccases (LCs) are multicopper oxidases that find application as versatile biocatalysts for the green bioremediation of environmental pollutants and xenobiotics. In this study we elucidate the degrading activity of Lac2 pure enzyme form Pleurotus pulmonarius towards aflatoxin B₁ (AFB₁) and M₁ (AFM₁). LC enzyme was purified using three chromatographic steps and identified as Lac2 through zymogra...

In an attempt to improve the salt tolerance of rice, we introduced katE, a catalase gene of Escherichia coli, into the indica rice cultivar Kasalath. Transformation was carried out using Agrobacterium tumefaciens strain EHA101 harboring a binary vector pIES6/Hm/katE which contains genes for catalase katE, hygromycine resistance gene HPT and kanamycin resistance gene NPTII in the T-DNA region. W...

Standardization of an efficient regeneration system for each strawberry genotype is generally an indispensible pre-requisite for the successful development of transgenic plants. In this paper, we review some key factors affecting the regeneration of strawberry plants via adventitious organogenesis or somatic embryogenesis, such as type of explant, growth regulators or dark/light treatments. Sin...

The cDNA encoding the 326 amino acids of a Class I basic chitinase gene from Leucaena leucocephala de Wit (KB3, Genbank accession: AAM49597) was cloned under the control of CaMV35S promoter in pCAMBIA 1300 and transferred to Koshihikari. Calli of Koshihikari rice was transformed with agrobacterium with this construct expressing the chitinase and βglucouronidase (GUS). The frequencies of calli 9...

Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be used in monocots, by presenting a system involving use of a novel infiltration solution (containing acetosyringone, cysteine, and Tween 20) that enables whole-plant level VIGS of (germinated) seeds in wheat and maize. Using the established system, phytoene...

Agrobacterium tumefaciens is a Gram-negative plant-pathogenic bacterium that causes crown gall disease by transferring and integrating its transferred DNA (T-DNA) into the host genome. We characterized the chromosomally encoded alpha-crystallin-type small heat-shock protein (alpha-Hsp) HspL, which was induced by the virulence (vir) gene inducer acetosyringone (AS). The transcription of hspL but...

Agrobacterium-mediated genetic transformation was explored for inducing insect resistance in mat rush (Juncus effusus L.). Firstly, the callus regeneration system was established, and secondly the Agrobacterium tumefaciens strain EHA105 with plasmid pKUB carrying cryIA(b), hph and gus genes, coding for hygromycin phosphotransferase and β-glucuronidase, respectively, was used for transformation....

A reproducible and highly efficient protocol for genetic transformation mediated by Agrobacterium has been established for greengram (Vigna radiata L. Wilczek). Double cotyledonary node (DCN) explants were inoculated with Agrobacterium tumefaciens strain LBA 4404 harboring a binary vector pCAMBIA 2301 containing neomycin phosphotransferase (npt II) gene as selectable marker, β-glucuronidase (GU...