Using an electrochemical nanobiosensor based on titanium carbide-carbon nanotubes polymeric nanocomposite for the epithelialovarian cancer early detection

Background & Aim: Ovarian cancer is the most lethal among female malignancies. So far, treatment improvements have affected patient survival, but it is still more important to provide an early diagnosis that can detect the disease in its early stages. Therefore, introducing a rapid, accurate, and low-cost method to disease detection can be important and necessary. Methods: This study introduced a rapid and accurate tool for ovarian cancer detection by its specific biomarker. For this purpose, after modifying the working electrode surface with nanocomposite, the specific single-stranded DNA probe was immobilized on its surface. Then the target sequence hybridization was investigated by measuring the electrochemical oxidation response of methylene blue. Results: Under optimal conditions, The DNA biosensor showed a linear range from 50pM to 500 nM with a low detection limit of 0.0012 pM. The DNA assay platform was also successfully used to detect target DNA added to blood serum, and its recovery was reported to be between 89.96 and 103.90%.  Conclusion: The designed electrochemical nanobiosensor can be used for the early detection of epithelial ovarian cancer with desirable sensitivity and efficiency.