The production of Yarrowia lipolytica lipase powder by improved spray-drying method

Lipase is used in the production of foods, flavor enhancers, detergents, cosmetics and pharmaceuticals. A common impediment to the production of commercial enzymes is their low-stability aqueous solutions. In this study, the downstream process was investigated to obtain a stable spray-dried lipase powder of Yarrowia lipolytica. The enzyme solution samples were supplemented with different concentrations of Arabic gum and milk powder to spray-drying. Samples were dried in a pilot spray dryer at inlet and outlet temperatures of 175 and 85 °C, respectively, at a flow rate of 15 liters per hour. The best lipase powder obtained from spray-drying with 3% of Arabic gum and 9% of milk powder formulation as compared with other formulations. Results showed that spray-dried lipase powders of Y. lipolytica had a good yield suitable for various applications.