The Effects of Nitric Oxide on Wound Healing in Burned Rats

Purpose: The exact role of nitric oxide (NO) in the wound has not been elucidated and the available reports are controversial. We tried to study the effects of NO on healing promotion of burns wound in rats. Materials and methods: 60 adult male rats weighing 250-270 gr. were used After induction of general anesthesia egion was exposed to 95c water for 8S in order to producing the wet burns. Then the animals divided into two categoriestegory have three proups: control, Expl which received 100mg/kg L-arginin intraperitonealy and Exp2 which received 10mg/kg L-NAME. Injections in the first category performed on the first, third and fifth days. Skin sampling was on seventh day after burn. Injections in the second category performed from the first to 13th day, every other day, and sampling was done on the 15th day. H&E, new epiderm and derm formation, the number of the blood vessels sections and sebaseous gland and neutrophil were counted in 10 areas of wounded increment by means of eye piece. Results: The rate of epiderm and derm formation the number of blood vessels and sebaseous gland in L- arginin groups were significantly higher than other groups. The number of neutrophils in L-NAME groups was significantly higher than other groups. Conclusion: The results showed that NO is effective in wound healing and with its induction during burn, probably it accelerates healing and prevents infection.