The Effects of Fibroblast Co-Culture and Activin A on in vitro Growth of Mouse Preantral Follicles
نویسندگان
چکیده مقاله:
Background: This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base medium as control group (n = 113), (ii) base medium supplemented with 30 ng/ml Activin A (n = 115), (iii) base medium co-cultured with mouse embryonic fibroblast (n = 113), and (iv) base medium supplemented with 30 ng/ml Activin A and co-cultured with fibroblast (n = 115). Rate of growth, survivability, antrum formation, ovulation, embryonic development and steroid production were evaluated. Analysis of Variance and Duncan test were applied for analyzing. Results: Both co-culture and co-culture + Activin A groups showed significant difference (P<0.05) in growth (on days 4, 6, and 8 of culture period) and survival rates. However, there was no significant difference in antrum formation, ovulation rate, and embryonic development of ovulated oocytes. There were significant differences (P<0.05) in the estradiol production on days 8, 10, and 12 between co-culture + Activin A and the control group. Progesterone production also was significant (P<0.05) in co-culture + Activin A group on days 6, 8, 10, and 12 compared to control group. Conclusion: Fibroblast co-culture and Activin A promoted growth and survivability of preantral follicles. However, simultaneous use of them was more efficient.
منابع مشابه
the effects of fibroblast co-culture and activin a on in vitro growth of mouse preantral follicles
background: this study was conducted to evaluate fibroblast co-culture and activin a on in vitro maturation and fertilization of mouse preantral follicles. methods: the ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-mem as based medium for 12 days. a total number of 456 follicles were cultured in four conditions: (i) base mediu...
متن کاملEffect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles
متن کامل
The effects of fibroblast co-culture and activin A on in vitro growth of mouse preantral follicles.
BACKGROUND This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. METHODS The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base mediu...
متن کاملeffect of fibroblast co-culture on in vitro maturation and fertilization of mouse preantral follicles
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متن کاملEffect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles
BACKGROUND The aim of this study was to evaluate fibroblast co-culture on in vitro maturation and fertilization of prepubertal mouse preantral follicles. MATERIALS AND METHODS The ovaries of 12-14 day old mice were dissected and 120-150 μm intact preantral follicles with one or two layers of granulosa cells, and round oocytes were cultured individually in α-minimal essential medium (α-MEM) su...
متن کاملThe Effect of Fibroblast Co-culture on In Vitro Maturation of Mouse Preantral Follicles
This study was performed to evaluate the effects of fibroblast co-culture on in vitro maturation (IVM) of prepubertal mouse preantral follicles. The intact preantral follicles were obtained from the ovaries of 12-14 day old mice and these were cultured individually in α-minimal essential medium (α-MEM) supplemented with 5% fetal bovine serum (FBS), 100 mIU/Ml recombinant follicle stimulating ho...
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عنوان ژورنال
دوره 18 شماره 1
صفحات 49- 54
تاریخ انتشار 2014-01
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