Survival Rate of Preantral Follicles Derived From Vitrified Mouse Ovarian Tissue by Cryotop
نویسندگان
چکیده مقاله:
Purpose: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by cryotop.Materials and Methods: Ovaries of 14-day-old NMRI mice were separated and divided into three groups fowling as: vitrified, toxicity tested and control groups. Ovaries in the experimental groups were immersed in equilibration and vitrification solution composed of ethylene glycol (EG), dimethylsulfoxide (DMSO) in HEPES-buffering tissue culture medium 199 (TCM 199) with human serum albumin(HSA), then in vitrification group ovaries loaded on cryotop and plunged into liquid nitrogen whereas in toxicity test group ovaries immediately were submitted to warming process. After three weeks the vitrified ovaries were warmed and Histological evaluation was performed. Preantral follicles from ovaries in three groups were isolated by mechanical dissection and isolated follicles were cultured for 4 days to compare survival rate and diameters of follicles between mentioned groups).Results: Survival rate in toxicity group (97.4%), alike the control group (98.7%) were significantly higher than the vitrification group (92.7%), (p<0.05). Increasing in follicles diameter after 4 days in vitrification group was significantly lower than the control and toxicity test groups (p<0.05).Conclusions: In spite of significantly difference in survival rate of preantral follicles between control and vitrification group, ovarian tissue vitrification by cryotop highly preserves the viability rate of the preantral follicles.
منابع مشابه
P-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles
Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...
متن کاملMaternal-Effect Gene Expression in Cultured Preantral Follicles Derived from Vitrified-Warmed Mouse Ovary
OBJECTIVE This study was conducted to assess survival of follicles, their oocyte maturation and fertilization potential as well as expression of early embryo developmental genes in in vitro cultured pre-antral follicles derived from vitrified-warmed mouse ovary. MATERIALS AND METHODS In this experimental study, ovaries of 12-day old Naval Medical Research Institute (NMRI) female mice were pla...
متن کاملmaternal-effect gene expression in cultured preantral follicles derived from vitrified-warmed mouse ovary
0
متن کاملOxygen consumption rate of early pre-antral follicles from vitrified human ovarian cortical tissue
The study of human ovarian tissue transplantation and cryopreservation has advanced significantly. Autotransplantation of human pre-antral follicles isolated from cryopreserved cortical tissue is a promising option for the preservation of fertility in young cancer patients. The purpose of the present study was to reveal the effect of vitrification after low-temperature transportation of human p...
متن کاملComparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method
BACKGROUND Cryopreservation of preantral follicles or ovarian tissues would enable the storage of large numbers of primordial follicles or preantral follicles and preserves the structural integrity of somatic and reproductive cells. In the present study, we compared the developmental potential of cryopreserved two-week-old mouse preantral follicles, ovarian tissue slices, two-week-old mouse ova...
متن کاملP-116: Maturation Genes Expression during In Vitro Culture of Vitrified Mouse Preantral Follicles
Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 5 شماره None
صفحات 0- 0
تاریخ انتشار 2008-12
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی برای این مقاله ارائه نشده است
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023