Stem Cell Bone Differentiation on Polyol Lactic Acid Composite Nanoparticles Containing 45S5 Bioactive Glass Nanoparticles

Abstract Background and Objectives Now day, using of stem cells and nanoparticles in the differentiation of stem cells is considered as a therapeutic approach. The purpose of this study was to synthesize and characterize nanocomposite polyacrylic polycarboxylic acid containing nanoparticles of biologically active glass 45S5 crushed and assessment effect of this composite on the propagation and growth of bone marrow derived mesenchymal stem cells in the external environment.   Materials and Methods In this experimental study, the bioactive glass nanoparticles were synthesized by planetary milling and coated on electrospun polyolactic acid nanofibers. The physicochemical and biological properties of the composite nanocarbon were evaluated by X-ray diffraction, scanning electron microscopy, MTT, acridine orange, and alkaline phosphatase assays.   Results The physicochemical properties showed that the structure of synthesis bioactive glass and the lactic acid poly lactic acid fibers were completely nano size and the uniform distribution of nanoparticles was well placed on the fiber. The cell analysis demonstrated the significant increase proliferation and differentiation of mesenchymal stem cells to bone cells.In the MTT assay, cell survival was expressed in the composite nanostructure after 7 days of cell culture (1.96 ± 0.2), while the control sample (0.76 ± 0.08).   Conclusions  According to the results of this study, the composite nanoscale composite is not cytotoxicity effects and has biocompatible. It is useful in bone tissue repair.