Sex determination methods in guppy (Poecilia reticulat)

Guppy, Poecilia reticulate, is a member of the Poecilidae family. This species is viviparous and one of the most popular ornamental fish in the world, whose phenotype is usually determined during the last ten days of pregnancy. However, most differentiation and development of secondary sexual traits are formed during adolescence, i.e. about 16 weeks after birth. These secondary characteristics that are used to identify gender include: body shape, size and color, tail and dorsal fin shape, and anterior fin deformity. Ornamental fish that have more color characteristics and attractiveness have a higher selling price commercially than their less attractive counterparts. Whereas males in guppies are usually more beautiful, colorful, brighter, and have longer fins than females. Separating them for investment purposes and management purposes is something that needs to be considered. Because, the production cost is the same for both sexes, but the demand for males is higher. Therefore, it is important to correctly identify males and females using inexpensive, accurate, effective and non-invasive methods.