Rapid purification of HU protein from Halobacillus karajensis

rapid purification of hu protein from halobacillus karajensis

the histone-like protein hu is the most-abundant dna-binding protein in bacteria. the hu protein non-specifically binds and bends dna as a hetero- or homodimer, and can participate in dna supercoiling and dna condensation. it also takes part in dna functions such as replication, recombination, and repair. hu does not recognize any specific sequences but shows a certain degree of specificity to ...

استخراج و تخلیص پروتئین hu از باکتری halobacillus karajensis

میکروارگانیسم ها برای فشرده کردن dna سلولی از گروهی از پروتئین های بازی استفاده می کنند. این گروه از پروتئین ها اثرات عمیقی بر روی بیان ژن ها، نوترکیبی، همانندسازی dna، رشد و توانایی حیات دارند و به عنوان پروتئین های شبه هیستونی شناخته می شوند. از جمله ی این پروتئین ها می توان به hu، h-ns، fis و اشاره کرد. پروتئین های hu برای نگهداری ساختار نوکلئوئید ضروری هستند. بعلاوه آنها در وظایف وابسته ب...

Halobacillus karajensis sp. nov., a novel moderate halophile.

A moderately halophilic, gram-positive, spore-forming bacterium was isolated from surface saline soil of the Karaj region, Iran. The strain, designated MA-2T, was strictly aerobic with rod-shaped cells that occurred singly, in pairs or short chains. It contained L-Om-D-Asp-type peptidoglycan and the major respiratory lipoquinone was MK-7. It was non-motile and had an ellipsoidal endospore locat...

Protein purification from whole tissues . Fatemeh Mehrpooyan*

Purification and characterization of antiviral protein from silkworm fecal matter

Antiviral proteins (AVP), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (NPV) in vitro and in vivo. The extract of silkworm fecal matter prepared in phosphate buffer solution of pH 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich AVP, then which was dialyzed. The dialysate was applied to the column containing silica gel-G, the column e...