Protective Effect of Human Menopausal Gonadotropins on Rat Ovarian Structure Following Use of Nandrolone Decanoate: From a Histological Standpoint

Background and Aim: Nandrolone decanoate is known as one of the most destructive environmental factors in the reproductive system in men and women. In this study, we assessed the protective effect of gonadotropins (hMG) on histological changes of rat ovarian structure. Materials and Methods: 50 female rats were divided into 5 groups, including control, nandrolone decanoate (ND), hMG, ND + hMG, and sham group (0.1 ml of olive oil and 0.1 ml sodium chloride 0.09%). After 4 weeks, qualitative histological studies were performed, and the total volume of ovarian vessels was estimated by stereological methods. Results: Nandrolone decanoate increased the mean volume of vessels (66%) and the use of hMG + ND led to an increased mean volume of vessels (83%) compared to the use of 0.1 ml of olive oil and 0.1 ml sodium chloride 0.09% (sham group) (P<0.001). Histological studies showed induction of ovarian cysts in ND treated group and the administration of hMG + ND decreased follicular damage. Conclusion: Nandrolone decanoate increased the total volume of ovarian vessels and induced histological changes in ovarian tissue. the Use of hMG with ND increased the total volume of ovarian vessels and prevented the harmful effects of ND on ovarian follicles.