Prolonged Laboratory Maintenance of Toxoplasma gondii Tachyzoites in Co-cultivation with the Bovine Theileria annulata-Infected Lymphoblastoids

Acidocalcisomes in Toxoplasma gondii tachyzoites.

Toxoplasma gondii tachyzoites were loaded with the fluorescent indicator fura 2 to investigate the transport mechanisms involved in maintaining their intracellular Ca2+ homoeostasis. The mitochondrial ATPase inhibitor oligomycin and the endoplasmic-reticulum Ca(2+)-ATPase inhibitor thapsigargin increased the intracellular Ca2+ concentration ([Ca2+]i), thus indicating the requirement for ATP and...

Immunogenicity of Gamma-Irradiated Toxoplasma gondii Tachyzoites in Mice

The Immunogenicity of γ-irradiated tachyzoites of a highly virulent strain of Toxoplasma gondii (RH strain) was investigated. Immunized mice survived challenge infection and displayed increased interferon gamma (INF-γ ) production. On the other hand, nonimmunized controls died after 10 days and displayed a significantly decreased lymphoproliferative response to Concanavalin A (Con A). No tachyz...

Characterization of HSP90 isoforms in transformed bovine leukocytes infected with Theileria annulata

HSP90 chaperones are essential regulators of cellular function, as they ensure the appropriate conformation of multiple key client proteins. Four HSP90 isoforms were identified in the protozoan parasite Theileria annulata. Partial characterization was undertaken for three and localization confirmed for cytoplasmic (TA12105), endoplasmic reticulum (TA06470), and apicoplast (TA10720) forms. ATPas...

The Therapeutic Value of Oxyteracycline Hydrochloride (Terramycin) in Cattle Infected Experimentally with Theileria annulata

Immune Responses of Mice Intraduodenally Infected with Toxoplasma gondii KI-1 Tachyzoites

Toxoplasma gondii Korean isolate (KI-1) tachyzoites were inoculated intraduodenally to BALB/c mice using a silicon tube, and the course of infection and immune responses of mice were studied. Whereas control mice, that were infected intraperitoneally, died within day 7 post-infection (PI), the intraduodenally infected mice survived until day 9 PI (infection with 1 × 10(5) tachyzoites) or day 11...

Biochemical, Hematological studies in cattle naturally infected with Theileria annulata

The aim of the present study dealt with biochemical and hematological changes in cattle which presented to the Azad clinic of veterinary school in Urmia during the period between Dec 2008 to January 2012. A total of 985 cattle with positive theileriosis were examined. Data were collected on hematological conditions of the cattle. Ages of these slaughtered buffaloes ranged from 6 to 9 years, (79...