Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs

Adenovirus polymerase chain reaction assay for rapid diagnosis of conjunctivitis.

PURPOSE To evaluate newly designed primers in a polymerase chain reaction (PCR) for the detection of adenovirus DNA in conjunctival swabs. METHODS Oligonucleotides were derived from the adenovirus hexon gene and modified such that a maximum of only two mismatches occurred with adenovirus types 2 through 5, 7, and 16. Specificity was determined against adenovirus types 2 through 4, 7, 8 throug...

Development and application of specific polymerase chain reaction assay targeting the gyrB gene for rapid detection of Riemerella anatipestifer.

A pair of PCR primers was designed and synthesized to amplify a gyrB gene sequence from Riemerella anatipestifer (RA). A fragment of 194 bp was detected in RA-positive isolates, whereas other isolates were negative, which confirmed the high specificity of the primers and PCR conditions. As little as 1.6 × 10(4) cfu/mL of cultural liquid was required by this method. We compared a 16S rRNA sequen...

Polymerase chain reaction method for the rapid detection of virulent Shigella spp.

Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction(PCR) test for detection of virulent Shigella spp.. For this purpose, the primers were designed to amplify a 526-bp internal region of the Shigella spp. icsA gene, which encod...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Characterization of Iranian Isolates of Canine Parvovirus in Fecal Samples Using Polymerase Chain Reaction Assay

Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms su...

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs.

The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that ...