Phytochemical Screening and Green Biosynthesis of Silver Nanoparticles Using Unripe Fruit of Ziziphus Vulgaris

In this present study, an environmentally friendly and rapid method for the synthesis of silver nanoparticles (SNPs) has been reported using Ziziphus Vulgaris (ZV) unripe fruit extract under mild conditions. The synthesized silver nanoparticles have been characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FT-IR) and ultraviolet-visible spectroscopy (UV–vis). Transmission electron microscopy analysis confirmed that the synthesized SNPs have spherical shape and their average size is about 20 nm. Moreover, antioxidant activity, total phenolic and flavonoid content of unripe fruit and seed were analyzed by DPPH free radical-scavenging, Folin Ciocalteu and aluminum chloride (AlCl3) assays, respectively. Different extracts of unripe fruit showed higher antioxidant activity, total phenol and flavonoid than seed of Ziziphus vulgaris. Moreover, antioxidant activity, total phenolic and flavonoid content of unripe fruit and seed were analyzed by DPPH free radical-scavenging, Folin Ciocalteu and aluminum chloride (AlCl3) assays, respectively. Different extracts of unripe fruit showed higher antioxidant activity, total phenol and flavonoid than seed of Ziziphus vulgaris.