Phylogenetic analysis of HSP70 gene of Aspergillus fumigatus reveals conservation intra-species and divergence inter-species
نویسندگان
چکیده مقاله:
Aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. The first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. The mainfocus of the present study is to perform the similarity, conservation and phylogeneticanalysis of heat shock protein 70 (HSP70) gene of the fungus A. fumigatus. Therefore,DNA sequence of HSP70 gene was obtained from a native airborne A. fumigatus.Similarity, divergence and conservation of HSP70 gene sequence were assayed andcompared to other strains and fungal species using nucleotide Basic Local AlignmentSearch Tool (BLAST), construction of phylogenetic tree and online analysis tools. Theresults revealed that the most similar aspergilli to the examined A. fumigatus strain wereA. fumigatus non-native strains, Aspergillus clavatus, Aspergillus niger, Aspergillusterreus, and Aspergillus nidulans. Among other fungal genera, Penicillium species werethe most similar fungal genus to A. fumigatus. Moreover, the alignment results revealedhigh levels of similarity and conservation of native A. fumigatus HSP70 gene sequencewith yeasts stress-Seventy subfamily A (SSA) gene sequence from HSP70 family ofgenes and lower similarities with other genes in the family (Ssb, Ssc, HscA, Kar2 andbipA). Penicillium spp. were the most similar fungi to Aspergillus species byphylogenetic analysis of HSP70 sequence. Other molds, yeasts and yeast-like fungi wereplaced in more distanced clades. In general, fungal HSP70 sequence analysis could directus to make a better understanding of thermo-tolerance in different fungal species.
منابع مشابه
phylogenetic analysis of hsp70 gene of aspergillus fumigatus reveals conservation intra-species and divergence inter-species
aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. the first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. the mainfocus of the present study is to perform the similarit...
متن کاملMitochondrial cytochrome b gene analysis of Aspergillus fumigatus and related species.
Nucleotide sequences of 426 bp from the mitochondrial (mt) cytochrome b genes of six anamorph species and two species of Neosartorya teleomophs of Aspergillus section Fumigati were determined. These sequences were used to build nucleotide- and amino acid-based trees for phylogenetic analysis. Thirteen strains of A. fumigatus including 10 clinical isolates of A. fumigatus, 1 type culture of A. f...
متن کاملAspergillus fumigatus-Related Species in Clinical Practice
Aspergillus fumigatus is the main etiologic agent of invasive aspergillosis (IA). Other Aspergillus species belonging to the section Fumigati (A. fumigatus complex) may occasionally be the cause of IA. These strains are often misidentified, as they cannot be distinguished from A. fumigatus by conventional morphological analysis and sequencing methods. This lack of recognition may have important...
متن کاملDYNAMIC COMPLEXITY OF A THREE SPECIES COMPETITIVE FOOD CHAIN MODEL WITH INTER AND INTRA SPECIFIC COMPETITIONS
The present article deals with the inter specific competition and intra-specific competition among predator populations of a prey-dependent three component food chain model consisting of two competitive predator sharing one prey species as their food. The behaviour of the system near the biologically feasible equilibria is thoroughly analyzed. Boundedness and dissipativeness of the system are e...
متن کاملGrowth inhibition of Candida species and Aspergillus fumigatus by statins.
Statins are a class of drugs widely used for lowering high cholesterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme in the synthesis of cholesterol. We studied the effects of two major statins, simvastatin and atorvastatin, on five Candida species and Aspergillus fumigatus. The statins strongly inhibited the growth of all species, except Candida krusei. ...
متن کاملPolyphasic taxonomy of Aspergillus fumigatus and related species.
The variability within Aspergillus fumigatus Fresenius and related species was examined using macro-, micro-morphology, growth temperature regimes and extrolite patterns. In addition, DNA analyses including partial beta-tubulin, calmodulin and actin gene sequences were used. Detailed examination of strains, considered as A. fumigatus earlier, showed that they could be divided into four groups i...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 2 شماره 2
صفحات 85- 96
تاریخ انتشار 2015-07-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023