Peroxisome proliferator-activated receptors (PPARα, PPARγ and PPARβ/δ) gene expression profile on ram spermatozoa and their relation to the sperm motility
نویسندگان
چکیده مقاله:
Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype has a distinct tissue distribution relating to the distinct functions. In this study, the mRNA abundance for PPARα, PPARγ and PPARβ/δ was evaluated and compared with high and low motile ram spermatozoa. Semen samples from 6 adult rams were fractionated on a two layer discontinuous Percoll gradient to high and low motile sperm and quantitative parameters of sperm motility were determined by CASA. Total RNA was extracted and the mRNA abundance for each gene was measured by relative quantification technique with Real time PCR. The levels of three isotypes of PPAR transcripts were significantly higher in high motile semen samples using quantitative RT-PCR. Some of sperm motility indices were also significantly correlated with PPARα and PPARγ relative expression. This study revealed the novel association of PPAR gene isotypes with sperm motility. Data from our study suggested PPARs are one of the possible factors that can be studied in male infertility.
منابع مشابه
Effect of Roasted Soybean and Canola Seeds on Peroxisome Proliferator-Activated Receptors Gamma (PPARG) Gene Expression and Cattle Milk Characteristics
The aim of this research was to investigate the changes in milk yield and its composition, fatty acid profile and peroxisome proliferator-activated receptors gamma (PPARG) gene expression in adipose tissue of Iranian Holstein cattle in response to feeding isonitrogenous and isocaloric diets but formulated based on different oilseeds (soybean or canola seeds). Twenty cows were tested ra...
متن کاملP-68: Adipkines Play Major Roles in Sperm Motility and Function in Ram
Background: Adipose tissue is not only an energy storage tissue but also an important endocrine organ that secretes protein hormones. Among them, the production and secretion of adiponectin is inversely correlated to the severity of obesity. In adipose tissue, adiponectin production is transcriptionally regulated by peroxisome proliferator-activated receptor-γ (PPAR-γ). Obesity is a major healt...
متن کاملEvaluation of the Relationship between Peroxisome Proliferator Receptors (PPARα, PPARγ, and PPARδ) Expression and Carcinoembryonic Antigen (CEA) in Patients with Colorectal Cancer
Introduction: Studies have shown that an increase in carcinoembryonic antigen (CEA) is associated with the progression of colorectal cancer and is considered a sensitive diagnostic factor for CRC. Moreover, the role of peroxisome proliferators (PPARs) has recently been considered in colorectal cancer. This study aimed to investigate the relationship between the expression level of PPARs and CEA...
متن کاملPeroxisome proliferator-activated receptors and their relevance to dermatology.
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily and are expressed in a variety of tissues including skin and cells of the immune system. They act as ligand-dependent transcription factors which heterodimerize with retinoid X receptors to allow binding to and activation of PPAR responsive genes. Through this mechanism, PPAR ligands can ...
متن کاملPeroxisome Proliferator-activated Receptors
Peroxisome proliferator-activated receptors (PPARs) are members of the ligand-activated nuclear hormone receptor superfamily of transcription factors that includes receptors for steroids, thyroid hormone, retinoic acid, and vitamin D. Upon activation in the cytoplasm, PPARs heterodimerize with retinoid X receptors (RXR) and form a complex that translocates to the nucleus and regulates gene expr...
متن کاملPeroxisome proliferator-activated receptors and cardiovascular remodeling.
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors that heterodimerize with the retinoid X receptor and then modulate the function of many target genes. Three PPARs are known: alpha, beta/delta, and gamma. The better known are PPAR-alpha and PPAR-gamma, which may be activated by different synthetic agonists, although the endogenous ligands are unknown. PPAR-alpha is invol...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 7 شماره 1
صفحات 27- 34
تاریخ انتشار 2016-03-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023