P-88: Assessing Expression Changes of Some Wnt Pathway Genes During Goat Early Embryonic Development

Background: The developmental competency of embryos is affected by several factors, including the developmental pathways and their elements. In mammalian species including goat, fertilized oocyte undergoes several divisions to form a structure called blastocyst. These events depend on the successful control of temporal and spatial expression of genes involved in genome activation. One of the critical mediator signaling events during embryogenesis is Wnt signaling pathway. This pathway possesses large number of proteins that can control and regulate the production of Wnt signals. A growing body of evidence suggests that, there is a correlation between the appropriate regulation of Wnt signaling and proper embryonic development. Materials and Methods: In order to assess the mRNA expression of Frizzled, β-catenin and C-myc gene in caprine embryos, in vitro matured caprine oocytes were chemically activated and Day 3, 7 and 14 embryos and also MII stage oocyte were used for real time PCR. Results: Frizzled and c-myc expression in D3 increased dramatically compared to other stage, however, after this stage is accompanied by a sharp decline in expression. But for β-catenin highest expression levels can be seen in the oocyte stage and after that until D14 is facing a decline. Conclusion: Base on literature review, it was expected that pattern of changes for β-catenin gene was correlate with the two other genes but it was not, then we suggested, wnt pathway in Capra species maybe non-canonical pathway.