P-70: Effect of Xylose on Epididymal Sperm Motility in Water Buffalo (Bubalus bubalis)

Background: In mammalian spermatozoa, motility is one of the most important parameters for evaluating the quality of the semen. From a functional point of view, motility is strongly related to the spermatozoa’s ability to manage its energy status, Thus, modulation of energy status could be translated into significant changes in motility patterns. The aim of the present study was to evaluate xylose sugar different levels, on buffalo epididymal sperm motility patterns with computer-assisted sperm analyzers (CASA). Materials and Methods: For this study buffalo bull testicles (15 pairs) were picked up in the march till September 2012 in Urmia local slaughterhouse and transported to the laboratory in a cool container (filled with 5oc ice pack).After adding 5 levels of xylose (1-3-5-10-15 mM), in TCM199 media with 10 percent BSA and ,24 hours incubation in 37ºC was performed. Sperm motility was examined at 1, 3, 5, 7, 10, 12 and 24 hours after incubation with CASA. After collection of data from CASA analysis, Statistical analyses were performed with procedures available in ANOVA of SPSS version 20. Results: The results showed that CASA parameters; rapid progressive motility (Class A, %), progressive motility (Class B, %), motile sperms (Class A+B+C, %), straight line velocity (VSL, μm/s), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s), amplitude of lateral head displacement (ALH, μm), angular displacement (MAD, D),beat cross frequency (BCF, Hz) were significantly higher in control than xylose levels specially in 24 hours after incubation (p<0.05). Conclusion: In conclusion, our results indicate that xylose, at concentrations from 1 to 15 mM, decrease CASA parameters, maybe xylose don’t activate the glycolytic pathway and ATP production at this concentrations in TCM 199 media in 37 ºC.