P-33: Expression of Toll-Like Receptor 2-3 Genes in Human Sertoli Cells

Background: Toll-like receptors (TLRs) constitute a major part of innate immunity, which can distinguish pathogen associate molecular pattern. Sertoli cells create a special immunological niche that protects somniferous tubules from auto antigens and pathogens. These cells are the only somatic cells in somniferous that protect testis cells against pathogens. The purpose of this study was to evaluate the expression of TLR 2 and TLR3 in human sertoli cells. Materials and Methods: Biopsies were obtained from 10 men who underwent TESE. All men taking part in this study had no history of infection and congenital disorders. Tissue samples were transferred to lectin coated Petri dishes after enzymatic dissociation and isolation. After few passages, all the cells were harvested and sorted by flowcytometery. Then, TLR gene expressions were determined by RT-PCR. Results: Isolation, purification and culture of the human sertoli cells were performed successfully. Also, it was shown that TLR 2 and TLR3 genes are expressed in these cells. In addition, it seems that TLR3 is expressed more than TLR2. Conclusion: These results showed that the expression of TLR genes in human sertoli cells specially TLR 2-3, could play an important role in developing immunity against pathogens as well as allo and auto-antigens in somniferous tubules.