P-240: Does Ciprofloxacin Exert Severe Oxidative Stress in Testicular Tissue?

Background: Ciprofloxacin was shown to have cytotoxic effects on testicular germ cells. Its mechanism of cytotoxic action is not fully understood. To investigate the possibility of the involvement of an oxidative stress induction in this mechanism, total antioxidant power (TAOP) in the testis was evaluated. Materials and Methods: A number of twenty four mature male NMRI mice were used.The animals assigned into control and test groups. The test groups subdivided into low dose (206 mg/kg) and high dose (412 mg/kg) CPFX treated groups. Control animals received carboxymetyl cellulose. All animals were treated orally for 45 days. At the end of the study the animals were sacrificed and testicular tissues were homogenized in PBS. TAOP of the testis was determined by measuring its ability to reduce Fe+3to Fe +2.The complex between Fe +2 and TPTZ gives a blue color with absorbency at 593nm. Results: Although TAOP in the testis of low dose and high dose treated mice slightly increased but this change is not significant among three groups. Conclusion: Oxidative stress induction by CPFX is not remarkable during 45 consecutive days. Therefore the direct action of CPFX may be involved in its mechanism of action on testicular germ cells. Meanwhile long exposure to CPFX (More than 45 days) may result in extend damages of germinal epithelium in testicular tissue which may in turn result in remarkable alterations in TAOC.