P-24: In Vitro Growth and Apoptosis Incidence, valuation of in Vitrified Human Ovarian Tissue Following Treatment with Growth Differentiating Factor 9B (GDF- 9B) and Leukemia Inhibitory Factor (LIF)

Background The conventional freezing and vitrification are different cryopreservation protocols for fertility preservation in cancer patients. The high effectiveness of vitrification for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. Follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after culture in the presence of GDF-9B and or LIF evaluated. MaterialsAndMethods Ovarian cortex biopsies from normal pregnant women fragmented and then divided to 2 main groups: vitrified and non-vitrified. Ovarian fragments in each main group cultured in 3 groups: in presence GDF-9B or LIF or absence any factor. Then the morphology, ultrastructure and incidence of apoptosis using biochemical analyses and analysis of apoptosis related genes expression in ovarian tissue fragments in all 6 experimental groups were evaluated before and after 2 weeks culture. Results Morphology and ultrastructure of vitrified human ovarian tissue were better preserved in vitrified group in presence of LIF compared to GDF-treated and non-treated groups.Apoptosis biochemical evaluations in all non-vitrified and vitrified treated groups were same. Apoptotic genes expression in both non-vitrified and vitrified groups at the end of culture period showed significant differences compared to non-treated groups. Morphological studies of ovarian tissue in all groups showed better conservation of ovarian follicles in presence of GDF- 9B and LIF treated groups (P