P-24: DGC-HOST Method: An Approach for Selection of Normal Sperm during ICSI

Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infertile men undergoing ICSI and compare clinical outcome with control group. Materials and Methods: Semen samples were obtained from 39 infertile couple with severe male factor. Oocytes of each partner were divided two group. DGC group that sperm selection were only based on concentration gradient; however in DGC-HOST group, sperm selection were based on both concentration gradient and HOST. Results: Our results showed that percentage of fertilization were higher in DGC-HOST compared to DGC group. But it was insignificant. In addition, percentage good embryo qualities on day 3 were significantly higher in DGC-HOST compare to DGC group. Conclusion: DGC-HOST procedure is a novel sperm selection method for infertile men with severe sperm parameters.