P-226: American Ginseng Effects on Damage Induced by Cyclophosphamide in The Nuclei of Rat Sperm

Background: Oxidative stress occurs when the oxidative homeostasis is damage. Excessive ROS are generated and this reaction has been proposed to produce marked damage to the structure and function of sperm. Materials and Methods: This study was designed to investigate the protective effects of of American Ginseng against cyclophosphamide - induced damage in the rat sperm. For this experiment, the mature male rats randomly divided into four groups, seven rats in each and were treatment with cyclophposphamide (6.1 mg/ kg body weight) and ginseng (500 mg/kg body weight) for 50 days. The animals were killed one day after the last treatment. Samples of spermatozoafrom the cauda epididymidis were dissected immediately. Acridin orange and Aniline blue staining used for DNA denaturation and chromatin quality assay. Significance of difference between two groups was evaluated using Tukey test. Results: In CP-treated group percentage of double stranded DNA and mature sperms decreased in the epididymal sperm as compared with the control group, while co-administration of ginseng decreased these alterations induced by CP.Conclusion: Alkaliting agents destroy the DNA and chromatin structure and function in sperm nuclei and this damage could be reversed by American Ginseng.