P-221: Impact of Prunus Cerasus on The Expression of HAS2 in Cumulus Cells on Mice

Background: Cherries are an important source of phyto- chemicals, especially anthocyanin. They have many potent biological Properties such as antioxidant activity. Oxidative stress is mainly insert their effect through decreasing of cell proliferation and viability. Infertility problem affect more than 15% of young couples in different societies. Oocyte expansion plays an important role in fertility and needs some factors such as hyaluronic acid. The objective of this research is to find if prunes’ cercus have an effect on the expression of HAS2 in Cumulus cells Materials and Methods: Thirty female bulbc/ mice aging 6-8 weeks, were superovulated with 10 unit hMG and 48 hours later 10 unit hCG as IP injection. For the collection of cumulus- oocyte complexes(COCs) mice were killed 36 hours later by cervical dislocation and COCs were collected from oviduct by flashing method .COCs were cultured in 20cc universal medium supplemented prunes’ cercus and overlaid with mineral oil and גּ with 20 incubated for 4-6 hours at 5% CO2 and 37°C. Surrounding cumulus cells were removed mechanically by using hyaluronidase .The cumulus cells was stored at- גּ 20 80°C until real-time PCR. PCR was performed by using a master mix containing CYBR green. The expansion level HAS2 of mRNA was evaluated by real-time PCR. Results: The result showed that in the presence of Prunus cerasus the expression of HAS2 gene is increased significantly in comparison to control group Conclusion: Prunus cerasus enhances the expression of HAS2 gene in cultured cumulus cells from bulbc/mice.