P-218: Investigation of Association between Angiotensin II Type 1 and 2 Receptor (AT1R & AT2R) Gene Polymorphisms and Susceptibility to Pre-Eclampsia (PE) in Iranian Women

Background: Hypertension during pregnancy period along with the presence of protein in the urine, after the 20th weeks of gestation is called preeclampsia (PE). About five percent of all pregnancies are manifested with PE and its exact etiology has still remained unknown. The renin-angiotensin system (RAS) has an important role in the regulation of blood pressure during pregnancy and so pathophysiology of PE. In this system, angiotensin II via two distinct receptors, angiotensin II type-1 (AT1R) and angiotensin II type-2 (AT2R) regulates blood pressure.Two single nucleotide gene polymorphisms (SNPs), including AT1R (+1166 A/C) and AT2R (+1675 G/A), which are associated with receptor function have been detected. Materials and Methods: This Case-control study was performed on 97 (25 severe and 72 mild) women with pre-eclampsia and 100 healthy normal Iranian women. Cases and controls were selected age & ethnic matched from the same geographic region (Fars province, south west of Iran). After DNA extraction, PCR-RFLP method was used for genotyping of AT1R at position +1166 A/C and AT2R at position +1675 G/A. Chi-square test was used for analysis the results. Results: Statistical analysis did not showed any significant differences between cases and controls regarding the distribution of genotype and allele frequencies at position +1166 A/C in AT1R or position +1675 G/A in AT2R gene ( p=0/64, p=0/4 respectively). Moreover no significant correlation between both polymorphisms and the severity of the disease was also observed. Conclusion: The results of the present study indicated that the genetic polymorphisms in AT1R and AT2R genes are not associated with susceptibility or severity of pre-eclampsia in south west Iranian women.