P-210: Estrogen Receptor Beta Gene Polymorphisms and Recurrent Pregnancy Loss: A Case- Control Study in A Population of Iranian Women

Background: Recurrent pregnancy loss (RPL), that affects 1-5% of couples, is a multifactorial disorder as both genetic and environmental factors are involved. The aim of this study was to determine association of single nucleotide polymorphisms (SNPs) located on estrogen receptor beta (ERβ) gene with the risk of RPL in a population of Iranian women; +1082G>A (rs1256049) in exon 5, +1730G>A (rs4986938) in exon 8, and rs1256030 C/T in intron 2. Materials and Methods: In this case-control study, two hundred and fifty women with a history of three or more consecutive pregnancy losses before 20th week of gestation and a hundred and five healthy women with at least two live births and no history of pregnancy loss were included. Genotyping was performed through polymerase chain reaction- restriction fragment length polymorphism analysis. Results: Among the three SNPs examined, there was a significant difference in the genotype distribution of the +1082G>A polymorphism; the GG, AG genotypes was different between the case and control groups (p< 0.05). However, we found no significant differences in the +1730G>A and rs1256030 C/T polymorphisms (χ(2) =0.07, p=0.421; χ(2) =0.233, p=0.895, respectively) between the subjects with RPL and controls. Conclusion: Our current findings suggest that +1082G>A and +1730G>A polymorphisms which are located on functional region of ERβ gene may influence pregnancy outcome in Iranian women with RPL.