P-2: Impact of Sesame Oil Supplementation onDiabetic Rat Epididymal Sperm

Background: Formation of hydrogen peroxide derivativesdue to metabolic disturbances in the male diabetics can affect their fertility. The antioxidant property of sesame oil has been documented, previously. The primary aim of the present study was to find if sesame oil supplementation can reduces the impact of diabetes on sperm parameters in rat or not. Materials and Methods: Eighteen numbers of male Wistar rats assigned to four experimental groups: Control (n=3); intact animals received no treatment, Diabetic rats (n=5); diabetic rats treated with 5 (n=5) and 10% (n=5) of sesame oil within their diet throughout 60 days of experiment. Diabetes induced by IV injection of 45 mg/kg streptozotocin dissolved in citrate buffer (PH= 4.5). After 60 days of experiment, the animals were euthanized and their testes-epididymis dissected and weighted by digital balance. Left cauda epidydimis were separated and total recovered sperms during 4 hrs of incubation in the normal saline (35- 37°C) were counted and divided by the weight of left testis and left epidydimis to find total sperm/g testis and epidydimis, respectively. Contra-lateral cauda epidydimis dissected from testis and a sample of sperm isolated in normal saline (35-37°C). Sperm progressive motility (SPM) of recovered sperms was analyzed subjectively with light microscopy and analyzing four different fields of slides. Sperm viability was analyzed with conventional stain Eosin B/nigrosin stain. Data analyzed by ANOVA and expressed as Lsmeans ± ESM.Results: Diabetes (228 ± 19.04 g) severely (p0.05). There was no significant difference between groups according to testes and epidydimis weights (p>0.05). Surprisingly, total sperm/g testis and epidydimis were not affected by diabetes (28.5 × 106) (p>0.05) compare to control (28.7 × 106) while they were severely influenced by sesame oil supplementation, either 5 (19.3×106) or 10 (14.1×106) %. Diabetes (19.4 ± 2.9) and 10 % supplementation of sesame oil (21 ± 2.9) severely affected (p