P-186: The Expression of IL-6Ra in Fallopian Tubes and Ectopic Pregnancy

Background: IL-6 is a proinflammatory and immunoregulatory cytokine to be very high in endometriosis and pelvic inflammatory disease, which are known risk factors for ectopic pregnancy(EP). The effects of IL-6 on target cells occur via the formation of a high-affinity complex with IL-6Ra that expressed in the cilia of the epithelial cells. In the present study,we compared expression of IL-6Ra in the human fallopian tube(FT) of women with ectopic pregnancy and normal pregnancy. Materials and Methods: In this study, a total of 24 FTs samples obtained from two groups including: 12 FTs of women that bearing an ectopic pregnancy(aged 22-35), 12 FTs of healthy pregnant women (aged 32-38). Ampullaris parts of Fallopian tubes were evaluated from specimens taken during tubal ligation in normal pregnancies or at operation for tubal surgery in ectopic pregnancies. Samples were processed for quantitative reverse transcriptase polymerase chain reaction to determine mRNA expressions of IL-6Ra. Results: IL-6Rα mRNA is expressed in in both the FT from women with ectopic pregnancies and normal pregnancies .Expression of IL-6Rα mRNAs was lower in fallopian tube from women with ectopic pregnancies than in normal pregnant women but it was not significant (p>0.05). Conclusion: There is not an association between expression of IL-6Rα and ectopic pregnancy. However, it was not possible to compare normal fallopian tube of women at comparable gestational age also it requires further study.