P-113: Survey of In Vitro Effect of Resveratrol on Nitric Oxide Secretion of Human Endometrial Epithelial Cells
نویسندگان
چکیده مقاله:
Background: Resveratrol is a natural polyphe- nolic compound, synthesized by plants as a phytoalexin and protects against ultraviolet radiation and fungal infection. Nitric oxide is formed from L-arginine through nitric oxide synthases (NOS), a group of enzymes that structurally resemble cytochrome P-450 reductase. In females, circulating NO is increased during follicle development and decreased immediately after ovulation. The aim of present study was to determine in vitro resveratrol effect on nitric oxide (NO) secretion by human endometrial epithelial cells. Materials and Methods: In this experiment study, human endometrial biopsies (n=8) were chopped in sterile conditions. Epithelial cells, were isolated after enzymatic digestion (collagenase 2 mg/ml) and cell filtration though filter mesh. Cell viability was defined by trypan blue staining. The cells were divided into five groups: control, 1,10, 20 and 50 μM resveratrol concentration for three culture periods(24,48,72 hours). Nitric oxide secretion were assessed by Geriss assay. Data was analyzed by one way ANOVA and p<0.05 was considered significant. Results: Difference between NO amount were significant in control, 1, 10, 20 and 50 μM resveratrol concentration were 9.08 ± 1.6, 9.62 ± 1.7, 11 ± 1.6, 12.78 ± 2.1, 20.2 ± 5.1 μM at 24- hour culture period (p=0.08), 11.94 ± 1.3, 13.61 ± 1.06, 15.91 ± 1.2, 16.64 ± 1.5, 25.63 ± 5.4 μM at 48 hours (p=0.04), 13.81 ± 1.83, 16.06 ± 1.14, 16.43 ± 1.08, 17.45 ± 1.6, 26.74 ± 5.6 μM at 72 hours (p=000) respectively. Conclusion: Resveratrol increased endometrial epithelial cells NO secretion in dose dependent manner. Its higher doses showed significant effect.
منابع مشابه
P-156: Survey of In Vitro Effect of Resveratrol on Apoptosis of Human Endometrial Epithelial Cells
Background: In apoptosis is physiological cell death which eliminates injured and old cells. Any disruption in apoptosis, leads to disease. Resveratrol is a natural polyphenol with estrogen-like, antioxidant and antiinflammatory properties; also it showed cell prolifratory and inhibitory effect. The aim of present study was to determine resveratrol effect on apoptosis of human endometrial epith...
متن کاملSildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro
متن کامل
P-96: Survey of In Vitro Effect of Noscapine on Nitric Oxide Secretion of Human Endometrial Stromal Cell
Background: Noscapine is cough suppressant drug with cell inhibitory effect. It is introduced for anti-cancer treatment. The aim of present study was to determine in vitro noscapine effect on nitric oxide (NO) secretion by human endometrial stromal cells. Materials and Methods: Human endometrial biopsies (n=8) were obtained in sterile condition and were chopped mechanically and digested by enzy...
متن کاملsildenafil effect on nitric oxide secretion by normal human endometrial epithelial cells cultured in vitro
0
متن کاملSildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro
BACKGROUND Sildenafil is a selective inhibitor of cyclic-guanosine monphosphat-specific phosphodiesterase type 5. It increases intracellular nitric oxide (NO) production in some cells. There are reports on its positive effect on uterine circulation, endometrial thickness, and infertility improvement. Endometrial epithelial cells (EEC) play an important role in embryo attachment and implantation...
متن کاملthe effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 8 شماره 2.5
صفحات 126- 126
تاریخ انتشار 2014-07-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023