P-1: Study The Anti-inflammatory Effect of 1- (1- (4-Chlorobenzoyl) -6 -methoxy-l-1H-indol -3-yl) butan -2 -one on

Background: Leukocytospermia is found in both fertile and infertile men with and without evidence of infections of the genital tract. However, despite extensive research done in this subject, the clinical implications of this condition remain unclear. Leukocytes are also commonly found in semen samples, and a level above 1×106/mL is considered pathologic according to WHO criteria. Non-steroidal antiinflammatory drugs (NSAIDs) are important therapeutic agents for the treatment of the inflammation related to large variety of pathologies. NSAIDs inhibit the cyclooxgenase (COX) activity resulting in decreased synthesis of prostagland in, leukotriene and thromoxane precursors. Several reports indicate that NSAIDs can prevent the development various human tumors, including colon, breast, lung, gastric, and esophageal neoplasias. Materials and Methods: Semen samples were obtained from Leukocytospermic infertile men. This study includes 37 subfertile male partners between July 2013 to April 2014, from couples who had been consulted the infertility clinic of the Babil hospital of maternity (Hilla city/IRAQ).The Semen specimens were divided into four fractions. First part was control, 0.5ml of liquefied semen mixed with 0.5ml fertiCult medium and incubated at 37° C for 30 minutes. Second part (antibiotic 1), 0.5 ml liquefied semen was mixed with 0.5ml fertiCult medium supplemented with 0.02 mg/ml antibiotic and incubated at 37°C for 30 minutes. Third part (antibiotic 2), 0.5ml liquefied semen was mixed with 0.5ml fertiCult medium supplemented with 0.04 mg/ml antibiotic and incubated at 37°C for 30 minutes. Fourth part (antibiotic 3), 0.5ml liquefied semen was mixed with 0.5ml fertiCult medium supplemented with 0.06 mg/ml antibiotic and incubate at 37°C for 30 minutes. After semen specimen treated in all fractions, they were examined and assessed for macroscopical and microscopical changes. Results: The treatment of low-level leukocytospermia with present antibiotic show significantly enhancement in the semen parameters among the treated patients. Low-level leukocytospermia resolved in good percentage of the treatment groups. Conclusion: The results of this study indicated that adding present antibiotic to the culture medium for in vitro sperm activation leads to an improvement in certain sperm function parameters.