O-30: Impact of Environment Tobacco Smoke Exposure on Oxidative Stress in Antral Follicle and Assisted Reproduction Outcomes

Background: Cigarette smoke contains many oxidants and may alter the human reproduction by inducing oxidative stress (OS) in both active and passive smokers. This study was designed to evaluation the effect of environment tobacco smoke (ETS) exposure on oxidative stress in follicular follicle and assisted reproduction outcomes. Materials and Methods: A prospective study was carried out on 236 infertile women who underwent assisted reproduction cycles. The ETS exposure was assessed using self-reported ETS exposure and cotinine level in follicular fluid. To evaluate the OS in FF malon-di-aldehyde (MDA) and total antioxidant capacity (TAC) were measured. The number of retrieved oocytes, rate of metaphase II stage oocytes, fertilization rate, good cleavage rate and no fragmented embryo rate were considered assisted reproduction outcomes. The results were adjusted for age, body mass index, duration and etiology of infertility. P-values of less than 0.05 were considered significant. Results: The MDA and TAC levels in FF were not related to both self-report number of weekly ETS exposure and cotinine levels in FF (p<0.05). Also, the number of retrieved oocytes, MII stage oocytes, fertilization rate, good cleavage rate and no fragmented embryo rate were not related to cotinine level and weekly ETS exposure. But in women who their cotinine levels in FF were lower and equal/ above 3.5ng/ml, the number of retrieved oocytes was higher (12.63 ±. 71 vs. 9.28 ± 1.11). The relationship between OS and cleavage rate (p<0.05) and the relationship between MII stage rate and TAC (p<0.05) were positively significant. Conclusion: The ETS exposure may alter assisted reproduction success influencing on number of available oocytes. Although, the OS in follicular environment affect ability of oocytes to reach specific cleavage stages at appropriate time intervals, it does not mediate poor assisted reproduction outcomes due to ETS exposure.