O-15: Motile Sperm Organelle MorphologyExamination Is Stricter than Tygerberg Criteria
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چکیده مقاله:
Background: The present study aimed to evaluate the correlation between the motile sperm organelle morphology examination (MSOME) and a well-knownsperm morphology classification (Tygerberg criteria). Materials and Methods: Semen samples (one per subject) were obtained from 97 men (mean age 36.7±6.0years) from an unselected group of couples undergoing infertility investigation and treatment. A portion of each semen sample was processed for MSOME and the remainder of the same sample was analysed by Tygerberg’s. For MSOME, the morphological evaluation was performed after semen capacitation. Spermatozoa were analysed at x8400 magnification by inverted microscope (Eclipse TE 2000U Nikon, Japan) equipped with Nomarski differential interference contrast optics, Uplan Apo x100 oil/1.35 Background lens and variable zoom lens (C-mount). A sperm cell exhibiting a normal nucleus (normal shape and chromatin content), as well as a normal acrosome, postacrosomal lamina, neck, tail and mitochondria, and no cytoplasm around the head was classified as morphologically normal. At least 200 spermatozoa were evaluated and the percentage of normal spermatozoa was determined. By Tygerberg’s strict criteria the fresh semen underwent morphological evaluation as described in the literature. For spermatozoa to be considered normal, the sperm head, neck, midpiece and tail must be normal. One observer, blinded to subject identity, performed the entirestudy (MSOME and Tygerberg’s). Normal form percentages by Tygerberg’s and by MSOME were treated as a continuous variable for analysis. The Wilcoxon matchedpairs signed-ranks test and the Spearman rank correlation test were used where appropriate. The significance level was set at p
منابع مشابه
Motile sperm organelle morphology examination is stricter than Tygerberg criteria.
The present study aimed to evaluate the correlation between the motile sperm organelle morphology examination (MSOME) and a well-known sperm morphology classification (Tygerberg criteria). For MSOME, spermatozoa were analysed at x8400 magnification by inverted microscope equipped with Nomarski differential interference contrast optics, Uplan Apo x100 oil/1.35 objective lens and variable zoom le...
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عنوان ژورنال
دوره 4 شماره 2
صفحات 76- 76
تاریخ انتشار 2010-05-01
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