Molecular identification and phylogenetic analysis of chronic bee paralysis virus in Iran

نویسندگان

  • Hesameddin Akbarein Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Mohammadreza Ghorani Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Mohammadreza Rezapanah Center of Excellence for Organic Agriculture and Department of Biological Control, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
  • Omid Madadgar Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Reza Khaltabadi Farahani Iranian Veterinary Organization, Tehran, Iran
  • Sedigheh Nabian Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده مقاله:

Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular identification. Three positive samples were detected by RT-PCR. All positive samples were confirmed by sequencing. The phylogenetic tree which displays the molecular relationship between the viruses of different Iranian geographic regions and references isolates was constructed. The Iranian isolates formed two distinct phylogenetic groups (Group 1 and Group 2). The IR-CPV-GMG-1, IR-CPV-GMG-2, IR-CPV-GMG-4, and IR-CPV-GMG-6 formed Group 1 and IR-CPV-GMG-3, IR-CPV-GMG-5, and IR-CPV-GMG-7 were in Group 2 as a distinct group. Iranian isolates in group 1 were similar to European and East Asian CBPVs. This research was the first phylogenetic analysis of CBPV in Iran. Further researches are needed to study the other aspects of this virus-like genetic characteristics and pathogenesis in Iran.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular identification and phylogenetic analysis of chronic bee paralysis virus in Iran

Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular ide...

متن کامل

Molecular detection of Acute Bee Paralysis Virus in Iran

Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. The aim of this study was evaluation of ABPV infection in honey bee colonies ...

متن کامل

First molecular detection of Chronic Bee Paralysis Virus (CBPV) in Iran

  Among the viruses infecting honey bees, chronic bee paralysis virus (CBPV) is known to induce significant losses in honey bee colonies. CBPV is an unclassified polymorphic single stranded RNA virus. Using RT-PCR, the virus infections in honey bees can be detected in a rapid and accurate manner. Bee samples were collected from 23 provinces of Iran, between July-September 2011 and 2012. A tota...

متن کامل

molecular detection of acute bee paralysis virus in iran

acute bee paralysis virus (abpv) is a small single stranded rna virus recently classified within the family dicistroviridae, genus cripavirus. here, we describe the first study of abpv in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. the aim of this study was evaluation of abpv infection in honey bee colonies ...

متن کامل

first molecular detection of chronic bee paralysis virus (cbpv) in iran

among the viruses infecting honey bees, chronic bee paralysis virus (cbpv) is known to induce significant losses in honey bee colonies. cbpv is an unclassified polymorphic single stranded rna virus. using rt-pcr, the virus infections in honey bees can be detected in a rapid and accurate manner. bee samples were collected from 23 provinces of iran, between july-september 2011 and 2012. a total o...

متن کامل

Molecular Identification of Chronic Bee Paralysis Virus Infection in Apis mellifera Colonies in Japan

Chronic bee paralysis virus (CBPV) infection causes chronic paralysis and loss of workers in honey bee colonies around the world. Although CBPV shows a worldwide distribution, it had not been molecularly detected in Japan. Our investigation of Apis mellifera and Apis cerana japonica colonies with RT-PCR has revealed CBPV infection in A. mellifera but not A. c. japonica colonies in Japan. The pr...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 8  شماره 4

صفحات  287- 292

تاریخ انتشار 2017-12-15

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023