Molecular-Friendly Histopathology

molecular-friendly histopathology

the rapidly expanding fields of pharmacogenomics and pharmacodiagnostics have presented the pathology laboratories with many challenges and opportunities. as custodians of patient tissues, these laboratories are in the logical position to perform biomolecular testing for proper management of patients. in order to meet these challenges, the pathology laboratories of the twenty-first century shou...

Histopathology in the light of molecular profiling.

The 2001 WHO classification distinguishes five variants (centroblastic, immunoblastic, plasmablastic, anaplastic and T-cell rich) and three subtypes (primary mediastinal, intravascular and primary effusion large B-cell lymphoma) of diffuse large B-cell lymphomas (DLBCLs).The recognition of the three subtypes as distinct disease entities can be considered as an advance in our understanding of th...

Epithelial Columnar Breast Lesions: Histopathology and Molecular Markers

Correlating thyroid cytology and histopathology: Implications for molecular testing.

BACKGROUND A gene expression classifier (GEC) has been advocated in management of some indeterminate nodules without surgery. We assessed the potential negative predictive value (NPV) of the GEC at our academic center. METHODS Retrospectively, all cytologically indeterminate fine-needle aspirates (FNAs) diagnosed by University of Iowa cytopathologists over a 3-year period were identified. His...

Molecular histopathology by spectrally reconstructed nonlinear interferometric vibrational imaging.

Sensitive assays for rapid quantitative analysis of histologic sections, resected tissue specimens, or in situ tissue are highly desired for early disease diagnosis. Stained histopathology is the gold standard but remains a subjective practice on processed tissue taking from hours to days. We describe a microscopy technique that obtains a sensitive and accurate color-coded image from intrinsic ...