Lectin Histochemistry Assessment of Seminiferous Epithelium Glycoconjugates in Mouse

Purpose: Plasma membrane glycoconjugates are important determinantsof differentiation and iteraction during spermtogenesis. Which entails miotic and meiosis proliferation, alternation in cell shape and so on. Hihg rates of them synthetized and present in acrosomes. With attention to such importancy, the present study has was designed to analyze glycoconjugates with special terminal sugar residues by meanes of lectin histochemistry. Materials and Methods: samples abtained from fifteen healthy fertile mice. After fixation and routine laboratory process, 5,µm sections prepared from paraffinian blocks. Lectin histochmistry was used to perform in situ characterization glycoconjugates present in mouse seminiferoustubules. Results: There wasnt any reaction in spermatogonia. Reaction to Soybean, Madura pamifera. Arachis hypogaea and Vicia villosa agglutinin lectins apeared in cytoplasm,plasmamembrane, preacrosomal granules in first spermatocyte and increased in early spermatids. This followed by reduction in late spermatids. There wasnt any affinity for Lotus tetragonolobus agglutinin in germ cells. Sertoli cells reacted with Maclura parnifera and Arachis hypogaea agglutinin lectins. Conclusion: As terminl sugars in a high quantity, galactose and N-acetylgalactosamine. in acrosome, cytoplasm and plasmamembrane have possible and important role in spermatogenesis. In late spermatid for protection these sugars may mask sialic acid and therefore diminish reactions. Acrosome was the most relevant lectin-labeled structure.