Iron Nanoparticles and Methionine Hydroxy Analogue Chelate in ovo Feeding of Broiler Chickens

The 440 fertile eggs, in a completely randomized design were divided into 11 treatments with four replicates and 10 eggs per each replicate. Treatments including: (T1; control; without injection), (T2; injected with 0.3 ml saline 9.0%; sham control), (T3, T4 and T5) 25, 75, 125 ppm Fe-Nano, (T6, T7 and T8) 50, 100, 150 ppm Fe-Nano-Alimet chelate, (T9, T10 and T11) 50, 100, 150 ppm Fe-Aliment chelate. On 10th day of incubation, 0.3 ml solution in ovo was injected into the egg yolk sac. Higher hatchability was found in controls, 25 ppm Fe- Nano, 100 ppm Fe-Nano-Alimet chelate and all levels of Fe-Alimet chelate treatments than other treatments. The egg weight was higher significantly in sham control, 25 and 75 ppm Fe-Nano, all levels of Fe-Nano-Alimet chelate and 150 ppm Fe-Aliment chelate treatments. There was significant increase in chick weight in controls, 25 and 75 ppm Fe-Nano, 100 ppm Fe-Nano-Alimet chelate and 150 ppm Fe-Aliment chelate in comparison with other treatments. Also chick body weight to egg weight ratio in controls, 25ppm Fe-Nano and 100ppm Fe- Nano-Alimet chelate was higher than in other treatments. Level of 25 ppm of Fe-Nano has shown the highest relative liver weight. Serum Fe content and liver was higher by using 25 ppm Fe-Nano, 100 ppm Fe-Nano- Alimet chelate and 150 ppm Fe-Aliment chelate than other treatments. By applying two treatments of 100 ppm Fe-Nano-Alimet chelate and 150 ppm Fe-Aliment chelate, chickens blood hemoglobin increased significantly compared with the other treatments. These results suggest that 25 ppm Fe-Nano, 100 ppm Fe-Nano-Alimet chelate and 150 ppm Fe-Alimet chelate improved embryonic growth and development.