Induction of traumatic brain and spinal cord injury models in rat using a modified impactor device
نویسندگان
چکیده مقاله:
Introduction: The use of standard rodent model, allows for the understanding of neuronal injury physiopathology and helping development of therapeutic strategies. Because of eliminating technical problems, we designed a modified impactor device with ability to induce different degrees according to kilodyne from very mild to very severe of spinal cord injury (SCI) and traumatic brain injury (TBI) models in rat. Methods: For standardization and determining of optimal performance of the device to induce varying injuries, 47 adult male Wistar rats were used, and 8 different forces were applied in spinal cord and brain tissues. Results: The hematoxylin and eosin and 2, 3, 5-triphenyltetrazolium chloride (TTC) results demonstrated that by increasing the level of forces, histological changes in the spinal cord and brain were significantly enhanced. Different injuries had significant effect on the Basso-Beattie-Brenham and elevated body swing test outcomes, and there were significant differences between groups in comparison with control group. Conclusion: Our results showed that the modified device could be valid to produce precise SCI and TBI models, goal to replicate SCI and TBI in humans as much as possible. However, it might be considered that aspects of SCI and TBI models are complicate and more examination is necessary.
منابع مشابه
Induction of Traumatic Spinal Cord Injury in Rat Using a Device Made in Tabriz University of Medical Sciences
Abstract Backgrond:Spinal cord injury (SCI) leads to a serious neurological condition, associating with sensory and motor dysfunctions as well as urinary infections. In the experimental situations, using a valid SCI model helps to understand pathophysiological mechanisms and better ascertainment of therapeutic interventions. Because contusion type of SCI occurs commonly in human, in the pr...
متن کاملTraumatic brain and spinal cord injury.
The management of pediatric head injuries has evolved over the past decade,and a number of significant advances have been made. Evidence-based guide-lines and algorithms for the management of severe pediatric head injuries have recently been published, and all pediatricians who care for children with severe head injuries should be familiar with these guidelines. It is hoped the guidelines will ...
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عنوان ژورنال
دوره 22 شماره 4
صفحات 228- 239
تاریخ انتشار 2018-12
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