In Vitro Maturation of Mouse Round Spermatid Cells Using FSH and Testosterone

the effects of fsh and testosterone on in vitro maturation of fresh and frozen-thawed mouse round spermatid

introduction: both initiation and maintenance of spermatogenesis are hormonally regulated by fsh and testosterone. in this study, the effects of fsh and testosterone on the in vitro maturation of fresh and frozen- thawed round spermatids were determind. material and methods: cell suspension was isolated from testis of nmri male mice (8-10 weaks old) and divided into two groups .the first part w...

Lepista sordida Water Extract Enhances the Maturation of Mouse Dendritic Cells in Vitro and in Vivo

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

The Effect of Granulosa Cells Co-culture and Retinoic Acid on Maturation and Development of Immature Mouse Oocytes in Vitro

Purpose: The purpose of this study was to develop an appropriate medium for in vitro maturation (IVM) of immature mouse oocytes. Materials and Methods: Germinal vesicle of female NMRI mouse oocytes (6-8 weeks old) were collected from ovaries and cultured in maturation medium MEM-a, supplemented with: 100 mlIU/ml rFSH+7.5 IU/ml hCG+5% FCS (Control group) and 2mM all- trans retinoic acid (t-RA) ...

P-87: High Fat Diet Affects In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes

Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...

FSH and testosterone signaling in Sertoli cells.

Testosterone and follicle-stimulating hormone (FSH) are required to obtain full reproductive potential. In the testis, somatic Sertoli cells transduce signals from testosterone and FSH into the production of factors that are required by germ cells as they mature into spermatozoa. Recent advances in identifying new signaling pathways that are regulated by FSH and testosterone have allowed for re...