In Vitro induction of 3-hydrogenkwadaphnin production from Denrostellera lessertii seedlings

Denrostellera lessertii (Thymelaceae) is a native plant of Iran, which grows in limited area of Iran. So far, different groups have reported the anticancer products of many species of this family. The antitumor and anti-metastatic properties of D.lessertii have also been well worked out in our group and the most active component, 3-Hydrogenkwadaphnin has been purified and its chemical structure was established for the first time. For clinical application of this new anticancer agent, first we need to speed up the plant growth rate or its natural propagation. In that respect we induced germination of seeds and callus production as a probable source of 3-Hydrogenkwadaphnin. The results of seed germination, callus production and 3-Hydrogenkwadaphnin production quantities under various experimental conditions will be discussed. The innermost layer is very hard to break and this may explain the lack of natural germination, we removed the three coats, after sterilization of seeds, and placed the remnant on the M.S and/or B5-free-hormone media and kept at 25±3?C in the dark room