In vitro Antifungal Effect of Aqueous Garlic (Allium Sativum) Extract and its Combination with Fluconazole Against Five Common Clinical Candida Isolated from Candidiasis Lesions
نویسندگان
چکیده مقاله:
Introduction: Increasing rate of candidiasis prevalence and consequently use of antifungal drugs as prophylactic and curative agents has led to the widespread emergence of resistant strains. Therefore this study was designed to evaluate the in vitro antifungal activity of an aqueous extract of garlic and the synergic effect of garlic extract with fluconazole against common clinical isolates of Candida species from patients with candidiasis. Methods: The antifungal activity of aqueous garlic extract was investigated in an in vitro system using standard broth microdilution method against five common clinically isolates of Candida species including C. albicans, C. tropicalis, C. glabrata (T.glabrata), C. parapsilosis, C. krusei and 3 standard strains of Candida. The synergic antifungal effect of garlic extract in combination with fluconazole was also determined. Results: The strongest activity of garlic extract was seen against Candida tropicalis (MIC=0.78mg/ml), C. glabrata (MIC=1.56mg/ml), and C. albicans (MIC=3.12mg/ml) respectively. C. krusei was the most resistant species against garlic extract (MIC 6.25mg/ml). The minimum inhibition concentration of fluconazole was reduced eight folds against C. tropicalis, 4 folds against C. albicans and C. glabrata, and 2 folds for other Candida species in the presence of 3.12 mg/ml garlic extract. In comparing means, the isolated colonies (CFU) in wells without garlic extract and CFU in other wells showed statistical significant differences for C. tropicals (P=0.0001), C. glabrata (P=0.001) and C. albicans (P=0.002). Conclusion: Candida species particularly resistant species such as Candida tropicalis and Candida glabrata are sensitive to aqueous extract of garlic, and combination of garlic extract with fluconazole in topical use could increase the efficacy rate of fluconazole.
منابع مشابه
In vitro activity of econazole in comparison with three common antifungal agents against clinical Candida strains isolated from superficial infections
Background and Purpose: Candida species are the most common organisms involved in superficial fungal infections, worldwide. Although econazole is among the most frequently used topical formulations for the treatment of candidiasis, no information is available regarding the susceptibility profiles of Candida species in Iran. Materials and Methods: In vitro susceptibility of 100 clinical Candida...
متن کاملthe effect of aqueous extract of garlic on formalin-induced pain in male rats
چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...
15 صفحه اولIn vitro antifungal activity of Zataria multiflora essential oil, fluconazole and ciclopirox olamine against nonalbicans Candida species isolated from recurrent vulvovaginal candidiasis
The aim of the present study was to investigate the antifungal activity of Zatariamultiflora essential oil, fluconazole and ciclopirox olamine against 44 non-albicansCandida isolates from patients with recurrent vulvovaginal candidiasis. A set of C.glabrata (29 strains), C. krusei (3 stains) and C. patapsilosis (2 strains) were studiedusing Clinical and Laboratory Standards Institute (CLSI) bro...
متن کاملEffect of Aqueous Garlic (Allium sativum L.) Extract on Acetylcholine and Isosorbide-Induced Relaxation of Isolated Aorta in Rat
The hypotensive effect of garlic has been well-documented in human subjects and animals. Since endothelial activity regulates vascular reactivity in physiological and pathophysiological conditions, the aim of the present study was to investigate the effect of garlic on endothelium-dependent and independent relaxation of rat aorta for elucidation of mechanism of the garlic anti-hypertensive effe...
متن کاملActivities of fluconazole in combination with terbinafine against non-albicans Candida species isolated from the patients with recurrent vulvovaginal candidiasis in comparison with ciclopirox olamine
Introduction: Recent epidemiological studies show that episodes due to non-albicans species of Candida (C) such as Candida tropicalis, C. glabrata, C, krusei, C. parapsilosis appear to be increasing in recurrent vulvovaginal candidiasis (RVVC). Increased use of the current antifungal drugs cause drug resistance among Candida species. In order to gain suitable antifungal therapy for this disease...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 14 شماره 3
صفحات 153- 161
تاریخ انتشار 2007-07-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023