Improved Protocol for Isolation of Genomic DNA from Leaf Tissues of Phyllanthus emblica Gaertn

Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA quality was determined. Drastic reduction of pHwas the primary cause for poor quality of DNA. However, high quality DNA isolation was achieved bystabilizing the pH by addition of NaOH during different stages of DNA isolation process. The present protocolyielding high quality intact DNA for genetic fingerprinting as well as for amplification of chloroplast genes formolecular analysis.